Regional and Cellular Patterns of reelin mRNA Expression in the Forebrain of the Developing and Adult Mouse
Regional and Cellular Patterns of reelin mRNA Expression in the Forebrain of the Developing and Adult Mouse
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DOI：
10.1523/jneurosci.18-19-07779.1998
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发表时间：
1998-10
期刊：
影响因子：
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通讯作者：
S. Alcántara;M. Ruiz;G. D’Arcangelo;F. Ezan;L. de Lecea;T. Curran;C. Sotelo;E. Soriano
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文献类型：
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作者：
S. Alcántara;M. Ruiz;G. D’Arcangelo;F. Ezan;L. de Lecea;T. Curran;C. Sotelo;E. Soriano
reelin基因编码一种细胞外蛋白质，这种蛋白质对层状脑区域的神经元迁移至关重要。为了深入了解Reelin的功能，我们进行了高分辨率原位杂交分析，以确定Reelin在小鼠前脑发育中的表达模式。我们还进行了双标记研究，包括钙结合蛋白，GAD 65/67，和神经肽，以表征神经元亚群表达reelin转录。在胚胎第10天及以后在前脑中检测到reelin表达，其分布与前脑区域化的前体模型一致。在间脑，表达仅限于横向和纵向域划定神经节之间的边界。在胚胎发育过程中，在Cajal-Retzius细胞的大脑皮层中检测到reelin，但在第I层的GABA能神经元中未检测到。在产前阶段，reelin也表达在嗅球，纹状体和限制核腹侧端脑，下丘脑，丘脑和顶盖前。在出生后的阶段，reelin转录逐渐消失，从Cajal-Retzius细胞，在同一时间，因为它们出现在整个新皮层和海马层分布的GABA能神经元的子集。在其他端脑和间脑区域，reelin表达在出生后期间稳步下降。在成人中，有显着的表达在嗅球和大脑皮层，在那里它被限制到共表达钙结合蛋白，钙视网膜蛋白，神经肽Y，和生长抑素的GABA能中间神经元的子集。这种复杂的细胞和区域表达模式与Reelin在脑发育和成人脑功能中具有多种作用一致。
The reelin gene encodes an extracellular protein that is crucial for neuronal migration in laminated brain regions. To gain insights into the functions of Reelin, we performed high-resolution in situ hybridization analyses to determine the pattern of reelin expression in the developing forebrain of the mouse. We also performed double-labeling studies with several markers, including calcium-binding proteins, GAD65/67, and neuropeptides, to characterize the neuronal subsets that express reelin transcripts. reelinexpression was detected at embryonic day 10 and later in the forebrain, with a distribution that is consistent with the prosomeric model of forebrain regionalization. In the diencephalon, expression was restricted to transverse and longitudinal domains that delineated boundaries between neuromeres. During embryogenesis,reelin was detected in the cerebral cortex in Cajal-Retzius cells but not in the GABAergic neurons of layer I. At prenatal stages, reelin was also expressed in the olfactory bulb, and striatum and in restricted nuclei in the ventral telencephalon, hypothalamus, thalamus, and pretectum. At postnatal stages, reelin transcripts gradually disappeared from Cajal-Retzius cells, at the same time as they appeared in subsets of GABAergic neurons distributed throughout neocortical and hippocampal layers. In other telencephalic and diencephalic regions,reelin expression decreased steadily during the postnatal period. In the adult, there was prominent expression in the olfactory bulb and cerebral cortex, where it was restricted to subsets of GABAergic interneurons that co-expressed calbindin, calretinin, neuropeptide Y, and somatostatin. This complex pattern of cellular and regional expression is consistent with Reelin having multiple roles in brain development and adult brain function.