Growth at elevated ozone or elevated carbon dioxide concentration alters antioxidant capacity and response to acute oxidative stress in soybean (Glycine max)
Growth at elevated ozone or elevated carbon dioxide concentration alters antioxidant capacity and response to acute oxidative stress in soybean (Glycine max)
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DOI：
10.1093/jxb/erq435
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发表时间：
2011-05-01
影响因子：
6.9
通讯作者：
Ainsworth, Elizabeth A.
中科院分区：
文献类型：
--
作者：
Gillespie, Kelly M.;Rogers, Alistair;Ainsworth, Elizabeth A.
大豆 (Glycine max Merr.) 在较高的二氧化碳浓度 ([CO2]) 或长期较高的臭氧浓度 ([O-3]；90 ppb) 下生长，然后暴露于急性 O-3 胁迫（200 ppb，持续 4 小时），以检验大气环境改变植物总抗氧化能力及其对急性氧化胁迫的反应能力的假设。在急性 O-3 治疗之前、之后立即和恢复期间对总抗氧化代谢、抗氧化酶活性和抗氧化转录本丰度进行了表征。在长期升高的 [O-3] 条件下生长会增加植物的总抗氧化能力，而在升高的 [CO2] 条件下生长会降低植物的总抗氧化能力。总抗氧化能力的变化与抗坏血酸含量的变化相匹配，但与酚类含量的变化无关。生长环境显着改变了抗氧化转录本和酶对急性 O-3 应激的反应模式。急性氧化应激后，立即发生转录重编程，使在[O-3]升高的植物中生长的抗氧化酶活性得以维持或增加。 [CO2]升高时的生长似乎增强了抗氧化酶对急性氧化应激的反应，但抑制和延迟了转录反应。这些结果提供了证据，表明生长环境改变了抗氧化系统、对急性氧化应激的立即反应以及植物恢复到初始抗氧化水平的时间。结果还表明，未来升高的 [CO2] 和 [O-3] 将对抗氧化系统产生不同的影响。
Soybeans (Glycine max Merr.) were grown at elevated carbon dioxide concentration ([CO2]) or chronic elevated ozone concentration ([O-3]; 90 ppb), and then exposed to an acute O-3 stress (200 ppb for 4 h) in order to test the hypothesis that the atmospheric environment alters the total antioxidant capacity of plants, and their capacity to respond to an acute oxidative stress. Total antioxidant metabolism, antioxidant enzyme activity, and antioxidant transcript abundance were characterized before, immediately after, and during recovery from the acute O-3 treatment. Growth at chronic elevated [O-3] increased the total antioxidant capacity of plants, while growth at elevated [CO2] decreased the total antioxidant capacity. Changes in total antioxidant capacity were matched by changes in ascorbate content, but not phenolic content. The growth environment significantly altered the pattern of antioxidant transcript and enzyme response to the acute O-3 stress. Following the acute oxidative stress, there was an immediate transcriptional reprogramming that allowed for maintained or increased antioxidant enzyme activities in plants grown at elevated [O-3]. Growth at elevated [CO2] appeared to increase the response of antioxidant enzymes to acute oxidative stress, but dampened and delayed the transcriptional response. These results provide evidence that the growth environment alters the antioxidant system, the immediate response to an acute oxidative stress, and the timing over which plants return to initial antioxidant levels. The results also indicate that future elevated [CO2] and [O-3] will differentially affect the antioxidant system.