Long-term effects of biochar amendment on rhizosphere and bulk soil microbial communities in a karst region, southwest China
Long-term effects of biochar amendment on rhizosphere and bulk soil microbial communities in a karst region, southwest China
复制标题
生物炭改良对中国西南喀斯特地区根际和土壤微生物群落的长期影响
DOI：
10.1016/j.apsoil.2019.04.017
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发表时间：
2019-08-01
影响因子：
4.8
通讯作者：
Zhang, Qinghai
中科院分区：
文献类型：
--
作者：
Cheng, Jianzhong;Lee, Xinqing;Zhang, Qinghai
添加生物炭 (BC) 广泛用于农业中以调理土壤。然而，BC添加对喀斯特地区土壤微生物群落多样性和组成的影响尚不清楚，特别是长期施用后。为了解决这一知识差距，开展了一项现场实验，以检查六年BC修正后土壤理化性质和微生物群落的变化。 BC 以四个水平 (w/w) 应用于中国西南喀斯特地区的钙质土壤：0%、1.0%、5.0% 和 10%。然后通过 Illumina MiSeq 平台上的 16S rRNA 基因测序研究根际和土壤中的细菌群落组成。添加BC增加了根际和土壤中的土壤pH值、总碳(TC)、总氮(TN)和总氢(TH)含量。此外，BC改良与土壤细菌群落组成和多样性的变化有关，特别是在较高的BC施用水平下。随着BC改良的增加，根际土壤中Gemmatimonadetes的相对丰度增加，而Bacteroidetes、Firmicutes和Cyanobacteria的相对丰度则减少。随着BC施用水平的增加，散装土壤中变形菌门和绿柔菌门的相对丰度增加，而拟杆菌门和疣微菌门的相对丰度则减少。典型对应分析表明，细菌群落组成与土壤特征相关，包括根际和土体土壤的 pH、TC、TN 和 TH 含量。重要的是，这些土壤参数的变化与BC的施用量密切相关。这些结果表明，长期施用BC通过调节土壤理化性质，显着影响喀斯特地区的土壤细菌群落特征。
Biochar (BC) addition is widely used in agriculture to condition soils. However, the effects of BC addition on soil microbial community diversity and composition in karstic regions are unclear, especially after long-term application. To address this knowledge-gap, a field experiment was established to examine changes in soil physicochemical properties and microbial communities following six years of BC amendment. BC was applied to calcareous soils in a karstic region of southwestern China at four levels (w/w): 0%, 1.0%, 5.0%, and 10%. Bacterial community composition was then investigated in both the rhizosphere and bulk soils by 16S rRNA gene sequencing on the Illumina MiSeq Platform. BC addition increased soil pH, total carbon (TC), total nitrogen (TN), and total hydrogen (TH) contents in the rhizosphere and bulk soils. In addition, BC amendment was associated with changes in soil bacterial community compositions and diversities, especially at higher BC application levels. The relative abundances of Gemmatimonadetes increased in rhizosphere soils with increasing BC amendment, while those of the Bacteroidetes, Firmicutes, and Cyanobacteria decreased. The relative abundances of Proteobacteria and Chloroflexi increased in bulk soils with increasing BC application levels, while those of the Bacteroidetes and Verrucomicrobia decreased. Canonical correspondence analysis indicated that bacterial community composition was related to soil characteristics including pH, TC, TN, and TH contents in both rhizosphere and bulk soils. Importantly, variations in these soil parameters were closely associated with BC application rates. These results indicate that long-term BC application significantly impacts soil bacterial community characteristics in karstic regions via modulation of soil physiochemical properties.