Kinases of the Focal Adhesion Complex Contribute to Cardiomyocyte Specification.
Kinases of the Focal Adhesion Complex Contribute to Cardiomyocyte Specification.
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局灶性粘附复合物的激酶有助于心肌细胞规范。
DOI：
10.3390/ijms221910430
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发表时间：
2021-09-28
影响因子：
5.6
通讯作者：
Ogle BM
中科院分区：
文献类型：
--
作者：
Robert S;Flowers M;Ogle BM
多能干细胞向心肌细胞的分化受到培养条件的影响，包括细胞外基质或它们生长的类似合成支架。然而，将支架与分化结果联系起来的分子机制尚不完全清楚。在这里，我们通过免疫荧光染色和质谱方法确定，小鼠多能干细胞的细胞外基质（ECM）参与通过粘着斑激酶激活经典无翼/整合（Wnt）信号通路的关键组分，以驱动心肌发生。发现这些激酶根据所接合的ECM的类型而被差异激活。这些结果开始解释了随着发育和体外模型系统的体内组织的不同ECM组成如何产生不同的成熟细胞类型，对于工程化组织的设计具有广泛的实用性。
Differentiation of pluripotent stem cells to cardiomyocytes is influenced by culture conditions including the extracellular matrices or similar synthetic scaffolds on which they are grown. However, the molecular mechanisms that link the scaffold with differentiation outcomes are not fully known. Here, we determined by immunofluorescence staining and mass spectrometry approaches that extracellular matrix (ECM) engagement by mouse pluripotent stem cells activates critical components of canonical wingless/integrated (Wnt) signaling pathways via kinases of the focal adhesion to drive cardiomyogenesis. These kinases were found to be differentially activated depending on type of ECM engaged. These outcomes begin to explain how varied ECM composition of in vivo tissues with development and in vitro model systems gives rise to different mature cell types, having broad practical applicability for the design of engineered tissues.
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影响因子：
8
作者：
van Laake, L. W.;van Donselaar, E. G.;Monshouwer-Kloots, J.;Schreurs, C.;Passier, R.;Humbel, B. M.;Doevendans, P. A.;Sonnenberg, A.;Verkleij, A. J.;Mummery, Christine L.
通讯作者：
Mummery, Christine L.
影响因子：
5.6
作者：
Tan, Tzu-Wei;Huang, Yuan-Lin;Tang, Chih-Hsin
通讯作者：
Tang, Chih-Hsin
影响因子：
37.8
作者：
Go, Alan S.;Mozaffarian, Dariush;Turner, Melanie B.
通讯作者：
Turner, Melanie B.
影响因子：
10.8
作者：
Kandasamy, Arulmozhi D.;Schulz, Richard
通讯作者：
Schulz, Richard
DOI：
10.1073/pnas.1307405110
发表时间：
2013-11-26
影响因子：
11.1
作者：
Seong, Jihye;Tajik, Arash;Wang, Yingxiao
通讯作者：
Wang, Yingxiao