Genome Wide Association Mapping in Arabidopsis thaliana Identifies Novel Genes Involved in Linking Allyl Glucosinolate to Altered Biomass and Defense.
Genome Wide Association Mapping in Arabidopsis thaliana Identifies Novel Genes Involved in Linking Allyl Glucosinolate to Altered Biomass and Defense.
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DOI：
10.3389/fpls.2016.01010
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发表时间：
2016
影响因子：
5.6
通讯作者：
Kliebenstein DJ
中科院分区：
文献类型：
--
作者：
Francisco M;Joseph B;Caligagan H;Li B;Corwin JA;Lin C;Kerwin RE;Burow M;Kliebenstein DJ
现代生物学中的一个关键限制是快速鉴定新鉴定的复杂表型的基因的能力。全基因组关联研究（GWAS）通过在全基因组水平上将表型与基因型关联起来，已经成为剖析自然变异的一种越来越重要的方法。最近的研究表明，拟南芥的防御代谢产物，烯丙基芥子油苷（GSL），可能提供直接的反馈调节，连接防御代谢输出的生长，植物的防御反应。然而，仍然需要确定这一过程的基础基因。为了开始更深入地理解调节外源烯丙基GSL改变生长和防御能力的机制，我们测量了一系列天然96 A.用50 μM烯丙基GSL饲喂的拟南芥品种。外源烯丙基GSL被专门引入到根和化合物运输到叶，导致广泛的遗传效应植物生物量和内源GSL积累。利用自然变异，我们进行了GWAS，以确定一些新的基因，可能控制烯丙基反应在各种植物过程。这是第一个实例，其中这种方法已被成功地用于开始解剖一个新的表型，以潜在的分子/多基因的基础。
A key limitation in modern biology is the ability to rapidly identify genes underlying newly identified complex phenotypes. Genome wide association studies (GWAS) have become an increasingly important approach for dissecting natural variation by associating phenotypes with genotypes at a genome wide level. Recent work is showing that the Arabidopsis thaliana defense metabolite, allyl glucosinolate (GSL), may provide direct feedback regulation, linking defense metabolism outputs to the growth, and defense responses of the plant. However, there is still a need to identify genes that underlie this process. To start developing a deeper understanding of the mechanism(s) that modulate the ability of exogenous allyl GSL to alter growth and defense, we measured changes in plant biomass and defense metabolites in a collection of natural 96 A. thaliana accessions fed with 50 μM of allyl GSL. Exogenous allyl GSL was introduced exclusively to the roots and the compound transported to the leaf leading to a wide range of heritable effects upon plant biomass and endogenous GSL accumulation. Using natural variation we conducted GWAS to identify a number of new genes which potentially control allyl responses in various plant processes. This is one of the first instances in which this approach has been successfully utilized to begin dissecting a novel phenotype to the underlying molecular/polygenic basis.