Opaque1 Encodes a Myosin XI Motor Protein That Is Required for Endoplasmic Reticulum Motility and Protein Body Formation in Maize Endosperm
Opaque1 Encodes a Myosin XI Motor Protein That Is Required for Endoplasmic Reticulum Motility and Protein Body Formation in Maize Endosperm
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Opaque1 编码玉米胚乳内质网运动和蛋白体形成所需的肌球蛋白 XI 运动蛋白
DOI：
10.1105/tpc.112.101360
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发表时间：
2012-08-01
期刊：
影响因子：
11.6
通讯作者：
Song, Rentao
中科院分区：
文献类型：
--
作者：
Wang, Guifeng;Wang, Fang;Song, Rentao
肌球蛋白由多基因家族编码，参与许多基本的生物学过程。然而，它们在植物中的功能仍然知之甚少。在这里，我们报告的玉米（玉米）opaque 1（o 1），它编码的肌球蛋白XI蛋白的功能特性。O 1是一种典型的玉米种子突变体，具有不透明胚乳表型，但玉米醇溶蛋白含量正常。与野生型相比，o 1胚乳细胞显示扩张的内质网（ER）结构和数量增加的较小，畸形的蛋白体。O 1基因是通过图位克隆分离的，编码植物肌球蛋白XI家族的一个成员（肌球蛋白XI-I）。在胚乳细胞中，O 1蛋白与粗糙ER和蛋白体相关。O 1尾结构域（C-末端644个氨基酸）的过表达显着抑制烟草（烟草）细胞的ER流。酵母双杂交分析表明，通过热休克蛋白70相互作用的蛋白质O 1和ER之间的关联。综上所述，本研究表明O 1通过影响内质网的形态和运动性来影响蛋白体的生物合成，最终影响胚乳质地。
Myosins are encoded by multigene families and are involved in many basic biological processes. However, their functions in plants remain poorly understood. Here, we report the functional characterization of maize (Zea mays) opaque1 (o1), which encodes a myosin XI protein. o1 is a classic maize seed mutant with an opaque endosperm phenotype but a normal zein protein content. Compared with the wild type, o1 endosperm cells display dilated endoplasmic reticulum (ER) structures and an increased number of smaller, misshapen protein bodies. The O1 gene was isolated by map-based cloning and was shown to encode a member of the plant myosin XI family (myosin XI-I). In endosperm cells, the O1 protein is associated with rough ER and protein bodies. Overexpression of the O1 tail domain (the C-terminal 644 amino acids) significantly inhibited ER streaming in tobacco (Nicotiana benthamiana) cells. Yeast two-hybrid analysis suggested an association between O1 and the ER through a heat shock protein 70-interacting protein. In summary, this study indicated that O1 influences protein body biogenesis by affecting ER morphology and motility, ultimately affecting endosperm texture.