Methylene Blue-Conjugated Hydrogel Nanoparticles and Tumor-Cell Targeted Photodynamic Therapy
Methylene Blue-Conjugated Hydrogel Nanoparticles and Tumor-Cell Targeted Photodynamic Therapy
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DOI：
10.1002/mabi.201000231
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发表时间：
2011-01-10
影响因子：
4.6
通讯作者：
Kopelman, Raoul
中科院分区：
文献类型：
--
作者：
Hah, Hoe Jin;Kim, Gwangseong;Kopelman, Raoul
通过微乳液聚合，在染料与单体缀合之后，制备亚甲基蓝缀合的聚丙烯酰胺纳米颗粒。纳米颗粒在溶液中具有50-60 nm的直径。这种缀合方法使得每个纳米颗粒的亚甲基蓝负载量大幅增加，并且还使从纳米颗粒中浸出的染料最小化。此外，通过改变染料量，可以控制染料含量，从而能够更精确地控制单线态氧的产生能力。纳米颗粒用F3肽包被，其在体外特异性靶向选定的肿瘤细胞9 L、MDA-MB-435和F98。此外，MTT实验表明，纳米粒子无暗毒性，但具有良好的PDT疗效，随着纳米粒子剂量和照射时间的增加。
Methylene blue-conjugated polyacrylamide nanoparticles are prepared through a microemulsion polymerization, after conjugation of the dye with a monomer. The nanoparticles have a 50-60 nm diameter in solution. This conjugation method enables a large increase in loading of methylene blue per nanoparticle and also minimizes dye leaching out of the nanoparticle. Furthermore, the dye content can be controlled by variation of the dye amount, enabling a more refined control of the singlet oxygen production ability. The nanoparticles are coated with F3 peptides, which give specific targeting to selected tumor cells, 9L, MDA-MB-435, and F98, in vitro. In addition, MTT assays reveal that the nanoparticles have no dark toxicity but excellent PDT efficacy increasing with the nanoparticle dose and irradiation time.