A systematic RNA interference screen reveals a cell migration gene network in C-elegans
A systematic RNA interference screen reveals a cell migration gene network in C-elegans
复制标题
DOI：
10.1242/jcs.03274
复制
发表时间：
2006-12-01
影响因子：
4
通讯作者：
Schwarzbauer, Jean E.
中科院分区：
文献类型：
--
作者：
Cram, Erin J.;Shang, Hongyu;Schwarzbauer, Jean E.
细胞迁移在胚胎发育和组织形态发生过程中是必不可少的。在秀丽隐杆线虫的性腺发育过程中，远端尖端细胞的迁移形成了两个u形的性腺臂。如果远端尖端细胞过早停止或遵循异常路径，则会导致畸形，并且在活的线虫中很容易看到异常。在这里，我们描述了第一个全面的体内RNA干扰筛选细胞迁移所需的基因。在这个无偏倚筛选中，我们通过光学显微镜系统地分析了16,758个rna干扰耗尽实验，并鉴定了远端尖端细胞迁移所需的99个基因。遗传和物理相互作用数据将这些基因中的59个连接起来，形成一个细胞迁移基因网络，定义远端尖端细胞在体内的迁移。
Cell migration is essential during embryonic development and tissue morphogenesis. During gonadogenesis in the nematode Caenorhabditis elegans, migration of the distal tip cells forms two U-shaped gonad arms. Malformation results if the distal tip cells stop prematurely or follow an aberrant path, and abnormalities are easily visualized in living nematodes. Here we describe the first comprehensive in vivo RNA interference screen for genes required for cell migration. In this non-biased screen, we systematically analyzed 16,758 RNA-interference depletion experiments by light microscopy and identified 99 genes required for distal tip cell migration. Genetic and physical interaction data connect 59 of these genes to form a cell migration gene network that defines distal tip cell migration in vivo.