Gonadal expression of c-kit encoded at the W locus of the mouse.
Gonadal expression of c-kit encoded at the W locus of the mouse.
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发表时间：
1990-12
期刊：
影响因子：
4.6
通讯作者：
K. Manova;K. Nocka;P. Besmer;R. Bachvarova
中科院分区：
文献类型：
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作者：
K. Manova;K. Nocka;P. Besmer;R. Bachvarova
最近，已经表明c-kit原癌基因在小鼠的白色斑点（W）基因座处编码。该基因的突变导致生殖细胞、一些造血细胞和黑色素细胞的耗竭。为了进一步确定c-kit在配子发生中的作用，我们检测了其在胎儿晚期和出生后卵巢以及出生后睾丸中的表达。通过RNA印迹分析，c-kit转录本在晚期胎儿卵巢中未检测到，但在出生时出现。相对含量在幼年小鼠卵巢中达到最大值，在成年小鼠卵巢中下降。c-kit转录物在分离的原始卵母细胞、生长中的卵母细胞和完全生长的卵母细胞以及排卵的卵中以增加的量存在。在早期2-细胞胚胎中几乎没有检测到，在囊胚中没有检测到。原位杂交显示，c-kit转录本在一些卵母细胞的晚期胎儿卵巢和所有卵母细胞（从原始到完全生长）在卵巢从少年和成年小鼠。从14日龄开始，在卵巢间质组织中也观察到表达。使用间接免疫荧光，c-kit蛋白检测到的原始，生长和完全生长的卵母细胞的表面上，以及在1-和2-细胞阶段的胚胎，很少留在囊胚。不同年龄小鼠睾丸的原位杂交分析表明，从6日龄开始，精原细胞中有表达。使用所提供的信息，通过确定一个给定的小管的生精上皮细胞的周期的阶段，并通过以下的年龄依赖性的标记，它的结论是，C-kit的表达期至少从早在A2型精原细胞通过B型精原细胞和进入preleptotene精母细胞。在检查的所有年龄标记间质细胞。在卵母细胞中的表达模式与卵母细胞的生长密切相关，在雄性生殖细胞中与生殖腺增殖密切相关。
Recently, it has been shown that the c-kit proto-oncogene is encoded at the white spotting (W) locus in mice. Mutations of this gene cause depletion of germ cells, some hematopoietic cells and melanocytes. In order to define further the role of c-kit in gametogenesis, we have examined its expression in late fetal and postnatal ovaries and in postnatal testis. By RNA blot analysis, c-kit transcripts were not detected in late fetal ovaries but appeared at birth. The relative amount reached a maximum in ovaries of juvenile mice, and decreased in adult ovaries. c-kit transcripts were present in increasing amounts in isolated primordial, growing and full-grown oocytes, as well as in ovulated eggs. Little was detected in early 2-cell embryos and none in blastocysts. In situ hybridization revealed c-kit transcripts in a few oocytes of late fetal ovaries and in all oocytes (from primordial to full-grown) in ovaries from juvenile and adult mice. Expression was also observed in ovarian interstitial tissue from 14 days of age onward. Using indirect immunofluorescence, the c-kit protein was detected on the surface of primordial, growing and full-grown oocytes, as well as on embryos at the 1- and 2-cell stages; little remained in blastocysts. In situ hybridization analysis of testes from mice of different ages demonstrated expression in spermatogonia from 6 days of age onward. Using information provided by determining the stage of the cycle of the seminiferous epithelium for a given tubule and by following the age dependence of labeling, it was concluded that the period of expression of c-kit extends from at least as early as type A2 spermatogonia through type B spermatogonia and into preleptotene spermatocytes. Leydig cells were labelled at all ages examined. The expression pattern in oocytes correlates most strongly with oocyte growth and in male germ cells with gonial proliferation.