Expression profile analysis of the low-oxygen response in Arabidopsis root cultures
Expression profile analysis of the low-oxygen response in Arabidopsis root cultures
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DOI：
10.1105/tpc.004747
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发表时间：
2002-10-01
期刊：
影响因子：
11.6
通讯作者：
Dennis, ES
中科院分区：
文献类型：
--
作者：
Klok, EJ;Wilson, IW;Dennis, ES
我们使用DNA微阵列技术来鉴定参与拟南芥根培养物低氧反应的基因。转移到低氧条件后，在不同时间（0.5、2、4和20 h）采集cDNA样品，对含有3500个cDNA克隆的微阵列进行筛选。一个统计工具包确定了210个差异表达的基因在四个时间点。主成分分析表明，0.5小时的反应包含一个显着不同的基因组的差异，在其他三个时间点的调节。差异表达的基因包括已知的厌氧蛋白以及转录因子、信号转导成分和编码以前不知道参与低氧代谢的途径的酶的基因。我们发现，具有相似表达谱的基因的调控区包含相似的序列基序，这表明由共同的调控因子组协调转录控制基因组。
We used DNA microarray technology to identify genes involved in the low-oxygen response of Arabidopsis root cultures. A microarray containing 3500 cDNA clones was screened with cDNA samples taken at various times (0.5, 2, 4, and 20 h) after transfer to low-oxygen conditions. A package of statistical tools identified 210 differentially expressed genes over the four time points. Principal component analysis showed the 0.5-h response to contain a substantially different set of genes from those regulated differentially at the other three time points. The differentially expressed genes included the known anaerobic proteins as well as transcription factors, signal transduction components, and genes that encode enzymes of pathways not known previously to be involved in low-oxygen metabolism. We found that the regulatory regions of genes with a similar expression profile contained similar sequence motifs, suggesting the coordinated transcriptional control of groups of genes by common sets of regulatory factors.