Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions
Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions
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DOI：
10.1007/978-1-62703-487-6_24
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发表时间：
2013-01-01
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影响因子：
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通讯作者：
de Gier, Jan-Willem
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文献类型：
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作者：
Hjelm, Anna;Schlegel, Susan;de Gier, Jan-Willem
优化膜蛋白在大肠杆菌中过度表达的条件及其随后的纯化通常是一个费力且耗时的过程。 Lemo21（DE3）菌株可以方便地仅使用一种菌株来确定膜蛋白的最佳表达强度，并且膜蛋白C末端与绿色荧光蛋白（GFP）融合，这极大地促进了用于功能和结构研究的高质量膜蛋白材料的生产。
Optimizing the conditions for the overexpression of membrane proteins in E. coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify the optimal expression intensity of a membrane protein using only one strain, and membrane proteins C-terminally fused to Green Fluorescent Protein (GFP) greatly facilitates the production of high-quality membrane protein material for functional and structural studies.