The wild mouse bone marrow has a unique myeloid and lymphoid composition and phenotype
The wild mouse bone marrow has a unique myeloid and lymphoid composition and phenotype
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野生小鼠骨髓具有独特的髓样和淋巴样组成和表型
DOI：
10.1093/discim/kyad005
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Muir A
中科院分区：
文献类型：
--
作者：
Muir A
小鼠骨髓作为成年小鼠白细胞的主要来源，在免疫功能和健康中起着核心作用。实验室小鼠提供了一种人类同源、基因可操作和可复制的模型，使高质量的免疫学研究成为可能。然而，最近的研究对实验室小鼠研究的可移植性提出了质疑，并提出将小鼠暴露于野生和自然环境中可能是进一步免疫学突破的关键。迄今为止，还没有研究对野生小鼠骨髓进行深入的细胞分析。本研究利用来自孤岛种群的野生小鼠（英国苏格兰五月岛），并进行流式细胞术和组织学分析，以表征野生小鼠骨髓中的髓系、淋巴系、造血祖细胞和脂肪细胞区室。我们发现，与实验室小鼠的骨髓相比，野生小鼠的骨髓在每一种被评估的细胞类型上都不同。一些主要的区别包括；B细胞室变小，浆细胞增多，KLRG1+ CD8+ T细胞比例增加，骨髓谱系中CD11b表达减少，嗜酸性细胞室增大5倍。我们得出的结论是，野生小鼠骨髓与实验室小鼠骨髓截然不同，具有多种表型，据我们所知，从未在实验室模型中观察到过。对这些独特特征的进一步研究可能会揭示新的免疫机制，并对免疫系统在自然环境中的作用有更深入的了解。
The murine bone marrow has a central role in immune function and health as the primary source of leukocytes in adult mice. Laboratory mice provide a human-homologous, genetically manipulable and reproducible model that has enabled an immeasurable volume of high-quality immunological research. However, recent research has questioned the translatability of laboratory mouse research into humans and proposed that the exposure of mice to their wild and natural environment may hold the key to further immunological breakthroughs. To date, there have been no studies providing an in-depth cellular analysis of the wild mouse bone marrow. This study utilized wild mice from an isolated island population (Isle of May, Scotland, UK) and performed flow cytometric and histological analysis to characterize the myeloid, lymphoid, hematopoietic progenitor, and adipocyte compartments within the wild mouse bone marrow. We find that, compared to laboratory mouse bone marrow, the wild mouse bone marrow differs in every cell type assessed. Some of the major distinctions include; a smaller B cell compartment with an enriched presence of plasma cells, increased proportions of KLRG1+ CD8+ T cells, diminished CD11b expression in the myeloid lineage and a five-fold enlargement of the eosinophil compartment. We conclude that the wild mouse bone marrow is dramatically distinct from its laboratory counterparts, with multiple phenotypes that to our knowledge have never been observed in laboratory models. Further research into these unique features may uncover novel immunological mechanisms and grant a greater understanding of the role of the immune system in a natural setting.
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影响因子：
3.7
作者：
Weiskopf K;Schnorr PJ;Pang WW;Chao MP;Chhabra A;Seita J;Feng M;Weissman IL
通讯作者：
Weissman IL
教师意见建议的环境正常化概括了实验室小鼠的成人免疫特征。
DOI：
--
发表时间：
2016
期刊：
影响因子：
--
作者：
D. Bogunovic
通讯作者：
D. Bogunovic
影响因子：
64.5
作者：
Mitroulis I;Ruppova K;Wang B;Chen LS;Grzybek M;Grinenko T;Eugster A;Troullinaki M;Palladini A;Kourtzelis I;Chatzigeorgiou A;Schlitzer A;Beyer M;Joosten LAB;Isermann B;Lesche M;Petzold A;Simons K;Henry I;Dahl A;Schultze JL;Wielockx B;Zamboni N;Mirtschink P;Coskun Ü;Hajishengallis G;Netea MG;Chavakis T
通讯作者：
Chavakis T
野生小鼠的存活率*
DOI：
--
发表时间：
1973
期刊：
影响因子：
--
作者：
R. Berry;M. Jakobson;G. Triggs
通讯作者：
G. Triggs
影响因子：
32.4
作者：
Sawai CM;Babovic S;Upadhaya S;Knapp DJHF;Lavin Y;Lau CM;Goloborodko A;Feng J;Fujisaki J;Ding L;Mirny LA;Merad M;Eaves CJ;Reizis B
通讯作者：
Reizis B