Suppressive effect of ethanol extract from passion fruit seeds on IgE production
Suppressive effect of ethanol extract from passion fruit seeds on IgE production
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DOI：
10.1016/j.jff.2017.02.030
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发表时间：
2017-05-01
影响因子：
5.6
通讯作者：
Sugahara, Takuya
中科院分区：
文献类型：
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作者：
Mizusaki, Ai;Nishi, Kosuke;Sugahara, Takuya
使用 U266 细胞筛选了一系列食品对 IgE 产生的抑制作用，IgE 是 I 型过敏反应的关键分子。经过筛选，百香果种子乙醇提取物 (PFS) 已成为具有 IgE 产生抑制作用的食品原料。实时RT-PCR分析表明，IgE产生抑制作用是由IgE基因表达下调引起的。此外，PFS 选择性抑制 IgE 产生，并且不影响小鼠脾细胞 IgG、IgA 或 IgM 的产生。白藜芦醇和白皮杉醇被鉴定为百香果种子中含有的生物活性成分。使用过敏性接触性皮炎小鼠模型评估口服 PFS、白藜芦醇和白皮杉醇的效果。两种二苯乙烯类化合物均显着抑制口服小鼠 IgE 的产生。总体结果表明，百香果种子是具有抗过敏作用的功能性食品开发的有前途的候选者。 (C) 2017 Elsevier Ltd. 保留所有权利。
An array of foodstuffs was screened for the suppressive effect on production of IgE, a key molecule in the type I allergic reaction, using U266 cells. An ethanol extract from passion fruit seeds (PFS) has emerged as a food material with the IgE production-suppressive effect as the result of screening. Real-time RT-PCR analysis revealed that the IgE production-suppressive effect was caused by downregulated IgE gene expression. In addition, PFS selectively suppressed IgE production and did not affect the production of IgG, IgA, or IgM by mouse splenocytes. Resveratrol and piceatannol were identified as bioactive ingredients contained in passion fruit seeds. The effect of oral administration of PFS, resveratrol, and piceatannol was evaluated using a mouse model of allergic contact dermatitis. Both stilbenoids significantly suppressed IgE production in orally administered mice. Overall results indicated that passion fruit seeds are a promising candidate with the antiallergy effect for development of functional foods. (C) 2017 Elsevier Ltd. All rights reserved.