LOCALIZATION OF TISSUE FACTOR IN THE NORMAL VESSEL WALL AND IN THE ATHEROSCLEROTIC PLAQUE
LOCALIZATION OF TISSUE FACTOR IN THE NORMAL VESSEL WALL AND IN THE ATHEROSCLEROTIC PLAQUE
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DOI：
10.1073/pnas.86.8.2839
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发表时间：
1989-04-01
影响因子：
11.1
通讯作者：
GORDON, D
中科院分区：
文献类型：
--
作者：
WILCOX, JN;SMITH, KM;GORDON, D
组织因子（TF）产生细胞，确定在正常人血管和动脉粥样硬化斑块的原位杂交和免疫组化使用特异性核糖核酸探针TF mRNA和多克隆抗体针对人类TF蛋白。正常乳内动脉和大隐静脉内皮细胞未见TF mRNA和蛋白表达。在正常血管中，发现TF在图尼卡中膜中存在的分散细胞以及血管周围的成纤维细胞样外膜细胞中合成。动脉粥样硬化斑块内含有大量合成TF mRNA和蛋白的细胞。巨噬细胞存在的泡沫细胞和单核细胞相邻的胆固醇裂缝含有TF mRNA和蛋白质，作为间充质出现内膜细胞。显著的TF蛋白染色被发现沉积在邻近胆固醇裂隙和坏死核心内的mRNA阳性细胞周围的细胞外基质中。这些结果表明，TF蛋白沉积在基质的坏死核心的动脉粥样硬化斑块可能有助于人类动脉粥样硬化血管的高血栓状态。
Tissue factor (TF)-producing cells were identified in normal human vessels and atherosclerotic plaques by in situ hybridization and immunohistochemistry using a specific riboprobe for TF mRNA and a polyclonal antibody directed against human TF protein. TF mRNA and protein were absent from endothelial cells lining normal internal mammary artery and saphenous vein samples. In normal vessels TF was found to be synthesized in scattered cells present in the tunica media as well as fibroblast-like adventitial cells surrounding vessels. Atherosclerotic plaques contained many cells synthesizing TF mRNA and protein. Macrophages present as foam cells and monocytes adjacent to the cholesterol clefts contained TF mRNA and protein, as did mesenchymal-appearing intimal cells. Significant TF protein staining was found deposited in the extracellular matrix surrounding mRNA-positive cells adjacent to the cholesterol clefts and within the necrotic cores. These results suggest that deposition of TF protein in the matrix of the necrotic core of the atherosclerotic plaque may contribute to the hyperthrombotic state of human atherosclerotic vessels.