Synovial Fluid Mediated Aggregation of Clinical Strains of Four Enterobacterial Species.
Synovial Fluid Mediated Aggregation of Clinical Strains of Four Enterobacterial Species.
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DOI：
10.1007/5584_2020_573
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发表时间：
2021
影响因子：
--
通讯作者：
Esteban J
中科院分区：
文献类型：
--
作者：
Macias-Valcayo A;Staats A;Aguilera-Correa JJ;Brooks J;Gupta T;Dusane D;Stoodley P;Esteban J
脓毒性关节炎和人工关节感染（PJI）通常与革兰氏阳性球菌相关，然而，最近观察到肠杆菌属的病例急剧增加。最近，多个研究小组报告葡萄球菌在滑液存在下迅速形成自由漂浮的聚集体。这些聚集体比它们的抗生素对应物对抗生素挑战的抵抗力相对更强，因此可能在关节感染的发病机制中发挥作用。虽然葡萄球菌聚集体一直是该领域的主要关注点，但尚不清楚滑液介导的聚集（SFMA）在革兰氏阴性肠杆菌（GNE）中的分布有多广泛。通过这项工作，我们已经评估了SFMA在临床GNE分离PJIs。将代表一系列抗生素敏感性的阴沟肠杆菌、大肠埃希菌、肺炎克雷伯菌和奇异变形杆菌菌株中的两种PJI临床菌株暴露于10%牛滑液上清液（BSF），使用相对简单、快速的半定量方法，使用成像读板仪。BSF在0.5小时内刺激两株E. Cloacidum和P.mirabilis以及一种大肠杆菌菌株。在奇异变形杆菌和大肠杆菌的一种菌株中，从0.5小时暴露到2小时暴露，聚集体的大小显著增加。相比之下，K. pneumoniae菌株在BSF中聚集。这些初步研究结果表明，聚集可以在GNE中迅速发生，但程度似乎是菌株和种属特异性的。需要进一步的工作来评估SFMA对抗生素耐受性、宿主先天免疫和生物膜建立的影响。
Septic arthritis and prosthetic joint infection (PJI) are conditions commonly associated with Gram-positive cocci, however, a drastic increase in cases derived from enterobacterial species has recently been observed. Recently it has been reported by multiple groups that staphylococci rapidly form free-floating aggregates in the presence of synovial fluid. These aggregates are comparatively more resistant to antimicrobial challenge than their planktonic counterparts, and thus may play a role in the pathogenesis of joint infection. While staphylococcal aggregates have been the primary focus of interest in the field, it is unclear just how widespread synovial fluid mediated aggregation (SFMA) is in Gram negative enterobacteria (GNE). Through this work we have evaluated SFMA in clinical GNE isolated from PJIs. Two PJI clinical strains each of Enterobacter cloacae, Escherichia coli, Klebsiella pneumonia and Proteus mirabilis strains representing a range of antibiotic susceptibilities were exposed to 10% bovine synovial fluid supernatant (BSF) using a relatively simple, quick semi-quantitative method using an imaging plate reader. BSF stimulated aggregation within 0.5 hr both strains of E. cloacae and P. mirabilis and one strain of E.coli. In one strain of P. mirabilis and E.coli, the size of the aggregates significantly increased from 0.5 to 2 hr exposure. In contrast, neither K. pneumoniae strain aggregated in BSF. These preliminary findings show that aggregation can occur quickly in GNE, but the extent appears strain and species specific. Further work is required to assess the impact of SFMA on antibiotic tolerance, host innate immunity and the establishment of biofilms.
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DOI：
10.1016/j.ymthe.2017.06.021
发表时间：
2017-09-06
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
Howlin RP;Cathie K;Hall-Stoodley L;Cornelius V;Duignan C;Allan RN;Fernandez BO;Barraud N;Bruce KD;Jefferies J;Kelso M;Kjelleberg S;Rice SA;Rogers GB;Pink S;Smith C;Sukhtankar PS;Salib R;Legg J;Carroll M;Daniels T;Feelisch M;Stoodley P;Clarke SC;Connett G;Faust SN;Webb JS
通讯作者：
Webb JS
影响因子：
14.2
作者：
Rodriguez-Pardo, D.;Pigrau, C.;Ariza, J.
通讯作者：
Ariza, J.
影响因子：
4.4
作者：
Nair, Rajeshwari;Schweizer, Marin L.;Singh, Namrata
通讯作者：
Singh, Namrata
影响因子：
2.7
作者：
Chiu, Li Qi;Wang, Wilson
通讯作者：
Wang, Wilson
影响因子：
14.2
作者：
Benito, N.;Franco, M.;Ariza, J.
通讯作者：
Ariza, J.