UBE2S is associated with malignant characteristics of breast cancer cells
UBE2S is associated with malignant characteristics of breast cancer cells
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DOI：
10.1007/s13277-015-3863-7
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Senga, Takeshi
中科院分区：
文献类型：
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作者：
Ayesha, Akter Khondker;Hyodo, Toshinori;Senga, Takeshi
泛素化对于各种生物过程至关重要，例如信号转导、细胞内运输和蛋白质降解。越来越多的证据表明，泛素化在癌症的发展中起着至关重要的作用。在本报告中，我们研究了泛素结合酶 E2S (UBE2S) 在乳腺癌中的表达和功能。免疫组织化学分析显示UBE2S在乳腺癌中高表达。 siRNA 消除 UBE2S 会导致肌动蛋白细胞骨架和粘着斑破坏。有趣的是，FAK Tyr397 的磷酸化对于整合素介导的信号转导很重要，通过 UBE2S 敲低显着降低。我们还表明，UBE2S 敲低抑制了乳腺癌细胞的恶性特征，例如迁移、侵袭和不依赖锚定的生长。我们的结果表明 UBE2S 可能成为乳腺癌治疗的潜在靶点。
Ubiquitination is essential for various biological processes, such as signal transduction, intracellular trafficking, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskeleton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.