A head-attachable device for injecting nanoliter volumes of drug solutions into brain sites of freely moving rats
A head-attachable device for injecting nanoliter volumes of drug solutions into brain sites of freely moving rats
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DOI：
10.1016/s0165-0270(01)00428-9
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发表时间：
2001-09-30
影响因子：
3
通讯作者：
Sharpe, LG
中科院分区：
文献类型：
--
作者：
Ikemoto, S;Sharpe, LG
我们描述了一种头戴式微泵注射系统，用于将纳升体积的药物溶液注入自由活动大鼠的离散脑区。使用微型步进电机，微泵喷射系统可以很容易地用商业供应建造。在校准微泵注射系统时，我们发现它将在1-小时内提供每次输液50nL的可靠容量，每1分钟输液一次。从活体测试中，我们还发现，大鼠很容易以固定的时间间隔向伏隔核自我注射多达100剂D-苯丙胺，这表明该系统可以在自由活动的大鼠身上提供恒定量的输液。它(1)很容易附着在植入的导轨上，(2)紧凑和耐用，(3)重量只有10克，(4)耐受性好，对动物没有明显的不适。该系统克服了目前使用的颅内自我给药系统的一些弱点。爱思唯尔科学公司出版。
We describe a head-mounted micropump-injection system designed for the infusion of nanoliter volumes of drug solutions into discrete brain regions of the freely moving rats. Using a miniature step motor, the micropump-injection system can be readily constructed from commercially available supplies. In calibrating the micropump-injection system, we found that it will deliver a reliable volume of 50 nl per infusion over a 1-h period, with an infusion given every 1 min. From in vivo testing, we also found that rats readily self-administered up to 100 infusions of D-amphetamine into the nucleus accumbens at regular intervals, suggesting that this system can deliver constant volumes of infusions over time in freely moving rats. It (1) attaches easily to an implanted guide, (2) is compact and durable, (3) weighs only 10 g, and (4) is well tolerated with no apparent discomfort to the animal. This system overcomes some of the weaknesses of currently used intracranial self-administration systems. Published by Elsevier Science B.V.