Amino acids promote human blastocyst development in vitro
Amino acids promote human blastocyst development in vitro
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DOI：
10.1093/humrep/16.4.749
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发表时间：
2001-04-01
影响因子：
6.1
通讯作者：
Englert, Y
中科院分区：
文献类型：
--
作者：
Devreker, F;Hardy, K;Englert, Y
在一些物种中，氨基酸培养基的补充已被证明有利于胚胎着床前的发育。这项随机研究分析了体外受精后获得的人类胚胎在存在或不存在氨基酸组合的情况下从2- 4细胞阶段到囊胚阶段的体外发育。129个人类胚胎随机分布在三种无血清化学定义的顺序培养基中：(i)在桑葚胚期之前使用含谷氨酰胺（Gln）的无葡萄糖厄尔平衡盐溶液（EBSS），并补充葡萄糖用于囊胚形成；（ii）含谷氨酰胺和非必需氨基酸（AA）的无葡萄糖EBSS用于卵裂期发育，并补充所有20种AA用于囊胚形成（Earle's + AA）；（iii）含有氨基酸的连续商业培养基（K-SCIM）。胚胎连续培养24 h。发育第6天，对囊胚进行差异标记，检测滋养外胚层、内细胞团细胞、有丝分裂和死亡细胞的数量。三种连续培养基的囊胚发育相似。AA显著提高了胚泡细胞总数，从earl’s + Gln组的61.8 +/- 4.2个增加到earl’s + AA组的99.3 +/- 8.4个和K-SCIM组的100.2 +/- 9.4个（P = 0.005）。这种增加在滋养外胚层和内细胞团系中均存在（P < 0.02）。Earle’s + AA组死细胞指数显著降低（P = 0.047）。
Supplementation of culture media with amino acids has been shown to benefit preimplantation embryo development in several species. This randomized study analysed the in-vitro development of human embryos obtained after IVF in the presence or absence of a combination of amino acids from the 2- to 4-cell stage to the blastocyst stage. A total of 129 human embryos was randomly distributed between three serum-free chemically defined sequential media: (i) glucose-free Earle's balanced salt solution (EBSS) with glutamine (Gln) prior to morula stage, supplemented with glucose for blastocyst formation; (ii) glucose-free EBSS with glutamine and non-essential amino acids (AA) for cleavage stage development, and supplemented with all 20 AA for blastocyst formation (Earle's + AA); and (iii) a sequential commercial medium containing amino acids (K-SCIM). Embryos were individually cultured for successive periods of 24 h. On day 6 of development, blastocysts were differentially labelled and the numbers of trophectoderm and inner cell mass cells, mitoses and dead cells were examined. Blastocyst development was similar for the three sequential media. The mixture of AA significantly increased total blastocyst cell numbers from 61.8 +/- 4.2 with Earle's + Gln to 99.3 +/- 8.4 with Earle's + AA and 100.2 +/- 9.4 with K-SCIM (P = 0.005). This increase was present in both the trophectoderm and inner cell mass lineages (P < 0.02). Furthermore, the dead cell index was significantly lower with Earle's + AA (P = 0.047).