HYPERBARIC-OXYGEN LIMITS INFARCT SIZE IN ISCHEMIC RABBIT MYOCARDIUM IN-VIVO
HYPERBARIC-OXYGEN LIMITS INFARCT SIZE IN ISCHEMIC RABBIT MYOCARDIUM IN-VIVO
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DOI：
10.1161/01.cir.88.4.1931
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发表时间：
1993-10-01
期刊：
影响因子：
37.8
通讯作者：
DOWNEY, JM
中科院分区：
文献类型：
--
作者：
STERLING, DL;THORNTON, JD;DOWNEY, JM
背景。 我们在心肌缺血和再灌注的开胸兔模型中探索了增加氧压改变坏死的能力。方法和结果。 左冠状动脉的一个分支被闭塞30分钟，然后再灌注3小时。 通过三苯基四唑染色测量梗塞并表示为缺血区的百分比。 未处理的兔子使用 1 atm 绝对压力的 100% 氧气进行通气。治疗动物暴露于2.5 atm绝对压力的高压氧中。 1.0-atm 对照心脏的缺血区出现 41.5+/-4.6% 的梗塞。 仅在缺血期间、仅在再灌注期间或在缺血和再灌注期间暴露于高压氧的动物与对照动物相比，梗塞面积明显较小（分别为 15.2+/-2.9%、14.5+/-3.7% 和 9.8%+/-2.7%；P 小于或等于 0.01），表明它们受到了该程序的保护。 再灌注开始后 30 分钟开始高压氧治疗时，没有看到保护作用 (35.8+/-3.8%)。结论。 我们得出的结论是，高压氧限制了再灌注兔心脏中的梗塞面积，并且当再灌注时开始高压氧时可以实现该效果。
Background. We explored the ability of increased oxygen pressure to modify necrosis in an open-chest rabbit model of myocardial ischemia and reperfusion.Methods and Results. A branch of the left coronary artery was occluded for 30 minutes followed by 3 hours of reperfusion. Infarction was measured by triphenyl tetrazolium staining and expressed as a percentage of the ischemic zone. Untreated rabbits were ventilated with 100% oxygen at 1 atm absolute. Treatment animals were exposed to hyperbaric oxygen at 2.5 atm absolute. The 1.0-atm control hearts developed 41.5+/-4.6% infarction of the ischemic zone. Animals exposed to hyperbaric oxygen during ischemia only, reperfusion only, or ischemia and reperfusion had significantly smaller infarcts with respect to control animals (15.2+/-2.9%, 14.5+/-3.7%, and 9.8%+/-2.7%, respectively; P less-than-or-equal-to .01), indicating that they had been protected by the procedure. When hyperbaric oxygen was begun 30 minutes after the onset of reperfusion, no protection was seen (35.8+/-3.8%).Conclusions. We conclude that hyperbaric oxygen limits infarct size in the reperfused rabbit heart and that the effect can be achieved when hyperbaric oxygen is begun at reperfusion.