Catalytic activity of an RNA domain derived from the U6-U4 RNA complex.
Catalytic activity of an RNA domain derived from the U6-U4 RNA complex.
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源自 U6-U4 RNA 复合物的 RNA 结构域的催化活性。
DOI：
10.1126/science.8272868
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Nadal-Ginard,B
中科院分区：
文献类型：
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作者：
Yang,JH;Cedergren,R;Nadal-Ginard,B
U6 RNA包含两个区域，这两个区域对于核前体信使RNA(Pre-mRNA)的正确剪接是必不可少的。比较了来自不同门的U6-U4 RNA复合体的二级结构，发现了一个类似于催化锤头RNA基序的保守结构域。虽然在哺乳动物的U6-U4 RNA复合体中没有检测到催化活性，但U6 RNA的两个核苷酸变化和U4 RNA的一个核苷酸变化赋予了该复合体的切割活性。此外，野生型复合体的高度保守的结构域，没有伴随的侧翼区域，切割了RNA底物，并显示出锤头状核酶的其他特征。文中还讨论了该结构在前mRNA剪接中的可能参与。
U6 RNA contains two regions that are essential for proper splicing of nuclear precursor messenger RNA (pre-mRNA). A comparison of putative secondary structures of the U6-U4 RNA complexes from different phyla revealed a conserved domain that is similar to the catalytic hammerhead RNA motif. Although no catalytic activity was detected in the mammalian U6-U4 RNA complexes, two nucleotide changes in U6 RNA and one in U4 RNA conferred cleavage activity to the complex. Furthermore, the highly conserved domain of the wild-type complex, without the accompanying flanking regions, cleaved an RNA substrate and exhibited other characteristics of the hammerhead ribozyme. The possible involvement of this structure in pre-mRNA splicing is also discussed.