An Inhibitory Role of Nitric Oxide in the Dynamic Regulation of the Blood-Brain Barrier Function
An Inhibitory Role of Nitric Oxide in the Dynamic Regulation of the Blood-Brain Barrier Function
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DOI：
10.1007/s10571-007-9139-z
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发表时间：
2007-02
影响因子：
4
通讯作者：
A. Yamauchi;S. Dohgu;T. Nishioku;H. Shuto;M. Naito;T. Tsuruo;Y. Sawada;Y. Kataoka
中科院分区：
文献类型：
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作者：
A. Yamauchi;S. Dohgu;T. Nishioku;H. Shuto;M. Naito;T. Tsuruo;Y. Sawada;Y. Kataoka
1. 本研究旨在探讨一氧化氮（NO）对小鼠脑毛细血管内皮细胞（MBEC4）血脑屏障（BBB）功能的影响。组胺（20 ~ 100 μM）诱导MBEC4细胞产生1.6 ~ 7 μM的NO，且呈剂量依赖性。在30 min的时间内，每10 min加入14 μM和28 μM NO溶液，荧光素钠对MBEC4细胞的渗透系数和罗丹明123在MBEC4细胞中的细胞蓄积量呈剂量依赖性增加。本研究表明NO增加脑毛细血管内皮细胞的通透性，抑制p -糖蛋白外排泵，提示NO在血脑屏障功能的动态调节中起抑制作用。
1. The present study aimed at elucidating the effect of nitric oxide (NO) on blood-brain barrier (BBB) function with mouse brain capillary endothelial (MBEC4) cells.2. Histamine (20–100 μM) evoked NO production (1.6–7 μM) in MBEC4 cells in a dose-dependent manner.3. The permeability coefficient of sodium fluorescein for MBEC4 cells and the cellular accumulation of rhodamine 123 in MBEC4 cells were increased dose-dependently by the addition of NO solutions (14 and 28 μM) every 10 min during a 30-min period.4. The present study demonstrated that NO increased the permeability and inhibited the P-glycoprotein efflux pump of brain capillary endothelial cells, suggesting that NO plays an inhibitory role in the dynamic regulation of the BBB function.