Beta-nerve growth factor measurements in mouse serum.
Beta-nerve growth factor measurements in mouse serum.
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小鼠血清中的β神经生长因子测量。
DOI：
10.1111/j.1471-4159.1986.tb13054.x
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发表时间：
1986
影响因子：
4.7
通讯作者：
Lakshmanan,J
中科院分区：
文献类型：
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作者：
Lakshmanan,J
采用竞争性β-神经生长因子放射免疫法测定小鼠血清β-神经生长因子水平。当雄性和雌性小鼠在单独的笼子中饲养至少7天后，它们的基础血清β-NGF水平一直保持在±2 ng/ml。然而，在雄性小鼠中，当它们每笼养5只时，血清β-NGF水平显著升高。通过将先前隔离的小鼠分组在一个笼子里20分钟，在它们被杀之前，证实了血清β-NGF水平的升高，这可能是由雄性间的攻击所介导的。在所有攻击性雄鼠中，血清β-NGF水平升高了两个数量级。β-神经生长因子放射免疫测定值也通过使用攻击性雄性小鼠血清的轴突生长生物测定系统进行了验证。总而言之，这两种测量技术都证实了小鼠血清β-神经生长因子水平根据动物处理条件的不同而发生显著变化。
Mouse serum β‐nerve growth factor (NGF) levels were measured using a newly developed competitive β‐NGF radioimmunoassay. The basal serum β‐NGF levels in male and female mice were consistently <2 ng/ ml when these animals were maintained in individual cages for at least 7 days before they were killed. However, in male mice, serum β‐NGF levels were significantly elevated when they were housed 5 per cage. The rise in serum β‐NGF levels, presumably mediated by intermale aggression, was confirmed by grouping previously isolated mice together in one cage for 20 min before they were killed. In all aggressive male mice, serum β‐NGF levels were elevated by two orders of magnitude. The β‐NGF radioimmunoassay values were also validated by a neurite outgrowth bioassay system using the serum of aggressive male mice. In summary, both measurement techniques confirm that mouse serum β‐NGF levels undergo marked changes depending on animal handling conditions.