Estrogen increases GAP-43 (neuromodulin) mRNA in the preoptic area of aged rats.
Estrogen increases GAP-43 (neuromodulin) mRNA in the preoptic area of aged rats.
复制标题
雌激素会增加老年大鼠视前区的 GAP-43（神经调节蛋白）mRNA。
DOI：
10.1016/0197-4580(96)00063-2
复制
发表时间：
1996
影响因子：
4.2
通讯作者：
Dorsa,DM
中科院分区：
文献类型：
--
作者：
Singer,CA;Pang,PA;Dobie,DJ;Dorsa,DM
雌激素已被证明影响脑神经元的生长、分化和存活，并调节涉及突触形成和连接的过程。这些营养作用随着年龄的增长而减弱，因为雌激素分泌下降。生长相关蛋白GAP-43被发现集中在轴突生长锥中，并与神经元的生长和再生有关。以前的研究已经确定，GAP-43的表达可以通过雌激素在发育和成年大鼠脑的视前区进行调节。本研究旨在确定GAP-43 mRNA的雌激素调节是否保留在老年大鼠脑中。年轻（3个月）和老年（24个月）大鼠卵巢切除，以消除内源性雌激素和GAP-43 mRNA的视前区进行了评估，使用原位杂交，比较雌激素和车辆治疗年龄组之间。结果表明，GAP-43 mRNA杂交信号的年龄相关性下降，可以恢复到与雌激素治疗的年轻动物相当的水平。
Estrogen has been shown to affect the growth, differentiation, and survival of brain neurons and to modulate processes involved in synapse formation and connectivity. These trophic effects are diminished with aging as secretion of estrogen declines. The growth associated protein GAP-43 is found concentrated in axonal growth cones and is implicated in neuronal growth and regeneration. Previous studies have established that expression of GAP-43 can be modulated by estrogen in the preoptic area of developing and adult rat brain. This study was undertaken to determine whether this estrogenic regulation of GAP-43 mRNA is retained in aged rat brain. Young (3 months) and aged (24 months) rats were ovariectomized to remove endogenous estrogen and GAP-43 mRNA in the preoptic area was evaluated using in situ hybridization to compare estrogen and vehicle treatments between age groups. The results demonstrate an age-related decline in GAP-43 mRNA hybridization signal that can be restored to levels comparable to that seen in young animals with estrogen treatment.