Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal.
Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal.
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DOI：
10.1371/journal.pone.0015365
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发表时间：
2010-12-15
期刊：
影响因子：
3.7
通讯作者：
Ishii S
中科院分区：
文献类型：
--
作者：
Seong KH;Akimaru H;Dai P;Nomura T;Okada M;Ishii S
Hedgehog（Hh）信号通过激活Cubitus interruptus（Ci）/Glioblastoma（Gli）转录因子家族在各种发育过程中起重要作用。在正确模式的形成过程中，CI活动受到多种机制的调节，包括加工、运输和降解。然而，它仍然是难以捉摸的，如何CI清楚地认识到强和中等的Hh信号。路杀动物（Rdx）诱导果蝇翅盘前部区域的Ci降解。在这里，我们报告说，RDX抑制CI活动由两种不同的机制。在前/后界附近接受强Hh信号的区域，Rdx通过释放Ci的importin α3抑制Ci的核输入。在该区域，Rdx负调控转录因子Knot/Collier的表达。在更远的前部区域接收中等水平的Hh信号，RDX诱导的Ci降解，如前所述。因此，两种不同的机制，其中Rdx负调控CI可能发挥重要作用，微调Hh的反应。
Hedgehog (Hh) signalling plays an important role in various developmental processes by activating the Cubitus interruptus (Ci)/Glioblastoma (Gli) family of transcription factors. In the process of proper pattern formation, Ci activity is regulated by multiple mechanisms, including processing, trafficking, and degradation. However, it remains elusive how Ci distinctly recognizes the strong and moderate Hh signals. Roadkill (Rdx) induces Ci degradation in the anterior region of the Drosophila wing disc. Here, we report that Rdx inhibited Ci activity by two different mechanisms. In the region abutting the anterior/posterior boundary, which receives strong Hh signal, Rdx inhibited the nuclear import of Ci by releasing importin α3 from Ci. In this region, Rdx negatively regulated the expression of transcription factor Knot/Collier. In farther anterior regions receiving moderate levels of Hh signal, Rdx induced Ci degradation, as reported previously. Thus, two different mechanisms by which Rdx negatively regulates Ci may play an important role in the fine-tuning of Hh responses.
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