Effects of α-amanitin on nuclear maturation of porcine oocytes in vitro
Effects of α-amanitin on nuclear maturation of porcine oocytes in vitro
复制标题
α-鹅膏蕈碱对体外猪卵母细胞核成熟的影响
DOI：
10.1530/jrf.0.0980195
复制
发表时间：
1993
期刊：
影响因子：
3.8
通讯作者：
S. Meinecke
中科院分区：
文献类型：
--
作者：
B. Meinecke;S. Meinecke
：研究了培养开始后不同时间（0、0.5、1、2、4、6、8、12 和 20 小时）暴露于这种异质核 RNA (hnRNA) 合成抑制剂的 α-鹅膏蕈碱对卵丘封闭和裸露猪卵母细胞的影响。进一步的目标是确定去除抑制剂后核进展的顺序。在培养开始后0、0.5或1小时向含有促性腺激素的培养基（TCM 199中的10 iu PMSG ml-1）添加10微克α-鹅膏蕈素ml-1可防止卵丘扩张，并且仅4.4、6.4和3.5%的卵母细胞发生生发囊泡破裂。通过延迟向培养基中添加药物2-8小时（2小时：34.9%、4小时：53.5%、6小时：46.9%、8小时：59.2%生发囊泡破裂），这种抑制作用大大降低，并且在卵母细胞移植后12或20小时后添加α-鹅膏蕈碱时，没有观察到对核成熟的抑制。当卵丘-卵母细胞复合物在无抑制剂的培养基中培养>或= 2小时，然后转移到补充有α-鹅膏蕈碱的培养基中时，在44小时培养结束时，在所有情况下都观察到完全的卵丘扩张。在添加有 α-鹅膏蕈碱的培养基中培养前卵母细胞的剥脱，或将浓度为 1.0 和 10.0 mg ml-1 的 α-鹅膏蕈素显微注射到卵质中，对核进展没有任何影响。（摘要截断为 250 字）
: The effects of alpha-amanitin on cumulus enclosed and denuded porcine oocytes exposed to this heterogeneous nuclear RNA (hnRNA) synthesis inhibitor at different times (0, 0.5, 1, 2, 4, 6, 8, 12 and 20 h) after the start of culture was investigated. A further objective was to determine the sequence of nuclear progression after removing the inhibitor. The addition of 10 micrograms alpha-amanitin ml-1 to a gonadotrophin containing medium (10 iu PMSG ml-1 in TCM 199) at 0, 0.5 or 1 h after the onset of culture prevented cumulus expansion, and only 4.4, 6.4 and 3.5% of oocytes underwent germinal vesicle breakdown. This inhibitory effect was considerably reduced by delaying the addition of the drug to the culture medium for 2-8 h (2 h: 34.9%, 4 h: 53.5%, 6 h: 46.9%, 8 h: 59.2% germinal vesicle breakdown), and no inhibition of nuclear maturation was observed when alpha-amanitin was added after 12 or 20 h following explantation of the oocytes. When cumulus-oocyte complexes were cultured for > or = 2 h in inhibitor-free medium and then transferred to medium supplemented with alpha-amanitin, full cumulus expansion was observed in all cases, at the end of the 44 h culture. Denudation of the oocytes before culture in either medium supplemented with alpha-amanitin or microinjection of alpha-amanitin into the ooplasm at concentrations of 1.0 and 10.0 mg ml-1 remained without any effect on nuclear progression.(ABSTRACT TRUNCATED AT 250 WORDS)