The first trimester human trophoblast cell line ACH-3P: a novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations--TNF-alpha stimulates MMP15 expression.
The first trimester human trophoblast cell line ACH-3P: a novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations--TNF-alpha stimulates MMP15 expression.
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DOI：
10.1186/1471-213x-7-137
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发表时间：
2007-12-19
影响因子：
--
通讯作者：
Desoye G
中科院分区：
文献类型：
--
作者：
Hiden U;Wadsack C;Prutsch N;Gauster M;Weiss U;Frank HG;Schmitz U;Fast-Hirsch C;Hengstschläger M;Pötgens A;Rüben A;Knöfler M;Haslinger P;Huppertz B;Bilban M;Kaufmann P;Desoye G
胎盘的滋养层由不同功能的亚群组成。它们通过分泌信号相互作用，从而形成自分泌或旁分泌调节环路。将人早孕(孕12周)原代滋养层细胞与人绒毛癌细胞系(AC1-1)融合，建立了早孕滋养层细胞系(ACH-3P)。ACH-3P的滋养层细胞标志物(细胞角蛋白-7、整合素、基质金属蛋白酶)的表达、侵袭能力和转录组与原代滋养层细胞极为相似。细胞形态、细胞遗传学和倍增时间与亲本AC1-1细胞相似。ACH-3P细胞中也存在绒毛滋养层细胞和绒毛外滋养层细胞等不同的滋养层细胞亚群，并可通过表达HLA-G进行免疫分离。HL A-G阳性的ACH-3P表现为假足和绒毛外滋养细胞标志物的强表达。胰岛素样生长因子II受体和人绒毛膜促性腺激素的高表达是绒毛外滋养细胞自分泌刺激的基础。我们认为ACH-3P是研究同基因滋养细胞亚群相互作用的一种工具。这些细胞特别适合研究滋养细胞功能的各个方面的自分泌和旁分泌调节。例如，肿瘤坏死因子-α对HLAG阳性的ACH-3P和外植体中的基质金属蛋白酶15有新的作用。
The trophoblast compartment of the placenta comprises various subpopulations with distinct functions. They interact among each other by secreted signals thus forming autocrine or paracrine regulatory loops. We established a first trimester trophoblast cell line (ACH-3P) by fusion of primary human first trimester trophoblasts (week 12 of gestation) with a human choriocarcinoma cell line (AC1-1). Expression of trophoblast markers (cytokeratin-7, integrins, matrix metalloproteinases), invasion abilities and transcriptome of ACH-3P closely resembled primary trophoblasts. Morphology, cytogenetics and doubling time was similar to the parental AC1-1 cells. The different subpopulations of trophoblasts e.g., villous and extravillous trophoblasts also exist in ACH-3P cells and can be immuno-separated by HLA-G surface expression. HLA-G positive ACH-3P display pseudopodia and a stronger expression of extravillous trophoblast markers. Higher expression of insulin-like growth factor II receptor and human chorionic gonadotropin represents the basis for the known autocrine stimulation of extravillous trophoblasts. We conclude that ACH-3P represent a tool to investigate interaction of syngeneic trophoblast subpopulations. These cells are particularly suited for studies into autocrine and paracrine regulation of various aspects of trophoblast function. As an example a novel effect of TNF-α on matrix metalloproteinase 15 in HLA-G positive ACH-3P and explants was found.