Ikaros SUMOylation: Switching out of repression
Ikaros SUMOylation: Switching out of repression
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DOI：
10.1128/mcb.25.7.2688-2697.2005
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发表时间：
2005-04-01
影响因子：
5.3
通讯作者：
Georgopoulos, K
中科院分区：
文献类型：
--
作者：
Arco, PGD;Koipally, J;Georgopoulos, K
Ikaros通过增强和抑制基因表达在淋巴细胞发育和稳态中起关键作用。在这里，我们表明，Ikaros与SUMO通路的组件相互作用，并在体内SUMO化。在Ikaros上鉴定了两个SUMO化位点，其同时修饰导致Ikaros抑制功能的丧失。Ikaros SUMO化破坏了其参与组蛋白脱乙酰酶（HDAC）依赖性和HDAC非依赖性抑制，但不影响其核定位到近着丝粒异染色质。这些研究揭示了一种新的动态方式，通过这种方式，Ikaros介导的基因阻遏被SUMO化控制。
Ikaros plays a key role in lymphocyte development and homeostasis by both potentiating and repressing gene expression. Here we show that Ikaros interacts with components of the SUMO pathway and is SUMOylated in vivo. Two SUMOylation sites are identified on Ikaros whose simultaneous modification results in a loss of Ikaros' repression function. Ikaros SUMOylation disrupts its participation in both histone deacetylase (HDAC)-dependent and HDAC-independent repression but does not influence its nuclear localization into pericentromeric heterochromatin. These studies reveal a new dynamic way by which Ikaros-mediated gene repression is controlled by SUMOylation.