Adaptation of the rat cardiac proteome in response to intensity-controlled endurance exercise
Adaptation of the rat cardiac proteome in response to intensity-controlled endurance exercise
复制标题
DOI：
10.1002/pmic.200800268
复制
发表时间：
2009-01-01
期刊：
影响因子：
3.4
通讯作者：
Burniston, Jatin G.
中科院分区：
文献类型：
--
作者：
Burniston, Jatin G.
耐力训练可以改善心脏功能并预防心脏病。啮齿动物强度控制跑步模型复制了人类的耐力运动，可用于研究心脏的分子适应。大鼠 (n = 6, 280 +/- 3 g) 进行运动测试以测量其峰值摄氧量（(V) 超过 dotO(2)peak），训练规定为 70-75% (V) 超过 dotO(2)peak，持续 30 分钟，每周 4 天。最终（V）超过 dotO(2)peak 测试 4 小时后分离心脏，并使用 2-D 凝胶差异分析与体重匹配的对照动物（n = 6, 330 +/- 2 g）进行左心室蛋白质组比较。通过使用 MASCOT (www.matrixscience.com) 针对 Swiss-Prot 搜索 MS 和 MS/MS 谱来鉴定蛋白质。在 6 周治疗方案中，dotO(2)peak 的平均 (V) 增加了 23% (p = 0.008)，并且 23 个凝胶点不同 (p
Endurance training improves cardiac function and protects against heart disease. The rodent intensity-controlled running model replicates endurance exercise in humans and can be used to investigate molecular adaptations in the heart. Rats (n = 6, 280 +/- 3 g) performed exercise tests to measure their peak oxygen uptake ((V) over dotO(2)peak) and training was prescribed at 70-75% (V) over dotO(2)peak for 30 min, 4 days/wk. Hearts were isolated 4 h after a final (V) over dotO(2)peak test and left ventricle proteomes compared to weight-matched control animals (n = 6, 330 +/- 2 g) using differential analysis of 2-D gels. Proteins were identified by searching MS and MS/MS spectra against Swiss-Prot using MASCOT (www.matrixscience.com). Average (V) over dotO(2)peak increased 23% (p = 0.008) over the 6-week regimen and 23 gel spots differed (p