Bovine plasma kininogens. III. Structural comparison of high molecular weight and low molecular weight kininogens.
Bovine plasma kininogens. III. Structural comparison of high molecular weight and low molecular weight kininogens.
复制标题
牛血浆激肽原。
DOI：
10.1093/oxfordjournals.jbchem.a130629
复制
发表时间：
1974
影响因子：
2.7
通讯作者：
T. Suzuki
中科院分区：
文献类型：
--
作者：
M. Komiya;H. Kato;T. Suzuki
建立了一种从用于分离高相对分子质量激肽原的牛新鲜血浆中分离纯化低相对分子质量激肽原的方法。纯化的LMW激肽原也能与这两种抗血清反应。因此，HMW和LMW激肽原的免疫学性质非常相似，它们的抗原决定簇似乎是相同的。用溴化氰处理纯化的HMW激肽原时，获得了一段含有激肽序列的片段，其中Kallidin被胰酶释放[EC 3.4.21.4]。该片段的氨基酸序列与先前用溴化氰法从纯化的LMW激肽原中获得的片段相同。此外，高分子激肽原和低分子激肽原的S羧甲基化衍生物的胰酶肽谱非常相似：前者有43个肽点，后者有30个肽点；28个肽点是两个激肽原共有的。因此，HMW激肽原似乎包含构成LMW激肽原的所有结构片段，但需要对两者的一级结构进行更详细的研究才能证实这一点。比较了HMW和LMW激肽原的理化性质，并讨论了差异。
A method was developed for the purification of low molecular weight (LMW) kininogen from the same batch of bovine fresh plasma used for the isolation of high molecular weight (HMW) kininogen.Purified HMW kininogen reacted with both HMW and LMW kininogen antisera prepared, respectively, from immunized rabbit serum. Purified LMW kininogen also reacted with both antisera. Thus, the immunological properties of HMW and LMW kininogens were very similar, and their antigenic determinant groups seem to be identical.On treatment of purified HMW kininogen with cyanogen bromide, a fragment was obtained containing a kinin peptide sequence, from which kallidin was liberated by trypsin [EC 3.4.21.4]. The amino acid sequence of this fragment was identical to that of a fragment previously obtained from purified LMW kininogen by cyanogen bromide treatment. Moreover, tryptic peptide maps of S-carboxymethylated derivatives of HMW and LMW kininogens were very similar: the former gave 43 peptide spots and the latter 30 peptide spots; 28 peptide spots were found to be common to both kininogens. Thus, HMW kininogen seems to contain all the structural segments constituting LMW kininogen, though more detailed studies on the primary structures of the two are required to confirm this.The physicochemical and chemical properties of HMW and LMW kininogens were compared and differences were discussed.