Selective detection of bacteria by a dielectrophoretic impedance measurement method using an antibody-immobilized electrode chip
Selective detection of bacteria by a dielectrophoretic impedance measurement method using an antibody-immobilized electrode chip
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DOI：
10.1016/j.snb.2005.12.027
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发表时间：
2006-11-24
影响因子：
8.4
通讯作者：
Hara, Masanori
中科院分区：
文献类型：
--
作者：
Suehiro, Junya;Ohtsubo, Akio;Hara, Masanori
本文介绍了一种结合抗原抗体反应的介电阻抗测量方法对特定细菌的选择性检测方法。作者先前已经展示了一种称为DEPIM（介电阻抗测量）和EPA-DEPIM（电渗透辅助DEPIM）的细菌检测技术，使用正介电力将悬浮中的细菌捕获到指间电极芯片上。研究还表明，利用抗原-抗体反应引起的凝集现象，可以实现对特定细菌的选择性DEPIM检测。在本研究中，证明了抗原抗体反应与DEPIM或EPA-DEPIM的其他组合。抗体分子固定在电极芯片上，然后用正电介质电泳（DEP）初步捕获细菌。细菌在正DEP力的作用下被吸引到电极间隙，最终与玻璃表面接触并与固定化抗体结合。通过适当调节DEP力与液体流动阻力之间的平衡，可以选择性地将目标细菌（大肠杆菌）保留在芯片表面，避免不必要的非特异性结合。保留的细菌用EPA-DEPIM电检测。该方法实现了对非目标菌混合悬浮液中目标菌的选择性检测。(c) 2005 Elsevier B.V.版权所有
This paper describes a selective detection method for specific bacteria by using a dielectrophoretic impedance measurement method in conjunction with an antigen-antibody reaction. The authors have previously demonstrated a bacteria detection technique called DEPIM (dielectrophoretic impedance measurement) and EPA-DEPIM (electropermeabilization-assisted DEPIM) using a positive dielectrophoretic force to capture bacteria in suspension onto an interdigitated electrode chip. It has also been demonstrated that a selective DEPIM detection of specific bacteria could be realized by taking advantage of agglutination phenomena, which were caused by an antigen-antibody reaction. In the present study, other combinations of the antigen-antibody reaction with DEPIM or EPA-DEPIM were demonstrated. Antibody molecules were immobilized on the electrode chip before the preliminary bacteria trapping by positive dielectrophoresis (DEP). The bacteria were attracted to the electrode gap under the action of the positive DEP force and finally brought into contact with the glass surface to be bound with the immobilized antibody. By appropriately adjusting the balance between the DEP force and the drag force caused by liquid flow, the target bacteria (Escherichia coli) could be selectively retained on the chip surface avoiding undesired non-specific binding. The retained bacteria were electrically detected by EPA-DEPIM. It was also confirmed that the proposed method realized selective detection of the target bacteria from a mixed suspension with nontarget bacteria. (c) 2005 Elsevier B.V. All rights reserved.