Simultaneous determination of eight β-lactam antibiotics in human serum by liquid chromatography-tandem mass spectrometry
Simultaneous determination of eight β-lactam antibiotics in human serum by liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/j.jchromb.2011.03.001
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发表时间：
2011-05-01
影响因子：
3
通讯作者：
Itoh, Yoshinori
中科院分区：
文献类型：
--
作者：
Ohmori, Tomofumi;Suzuki, Akio;Itoh, Yoshinori
建立了同时测定人血清中氨苄西林、头孢唑林、头孢吡肟、头孢美唑、头孢噻肟、多尼培南、美罗培南、哌拉西林等8种β-内酰胺类抗生素的液相色谱-串联质谱（LC-MS/MS）方法。使用 Waters Oasis (R) HLB 柱 (30 mg) 对样品进行固相萃取 (SPE)。使用与亲水性化合物兼容的高分辨率十八烷基硅胶柱进行色谱分离，使用含有 0.1% 甲酸-甲醇的 10 mM 甲酸铵水溶液梯度。通过电喷雾电离三重四极杆质谱仪 (MS/MS) 检测抗生素，并通过多反应监测模式进行定量。总运行时间为 13 分钟。除多尼培南外，β-内酰胺抗生素的校准线性范围为 0.1-50 μg/mL。定量下限为0.005-0.5μg/mL，使用50μL血清。除多尼培南 (49.1%) 和美罗培南 (62.3%) 外，这些分析物的回收率均超过 80.2%。本方法适用于临床实践中β-内酰胺类抗生素的常规治疗监测。 (C) 2011 Elsevier B.V. 保留所有权利。
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of eight beta-lactam antibiotics, including ampicillin, cefazolin, cefepime, cefmetazole, cefotaxime, doripenem, meropenem, and piperacillin, in human serum. Sample specimens were subjected to solid phase extraction (SPE) using Waters Oasis (R) HLB cartridges (30 mg). Chromatographic separation was performed with a high-resolution octadecyl silica column compatible with hydrophilic compounds, using a gradient of 10 mM aqueous ammonium formate containing 0.1% formic acid-methanol. Antibiotics were detected by a triple quadrupole mass spectrometer (MS/MS) with electrospray ionization and quantified by the multiple reaction monitoring mode. A total run time of 13 min was applied. Linearity in the calibration was obtained over a range of 0.1-50 mu g/mL of the beta-lactam antibiotics, except for doripenem. The lower limit of quantification was 0.005-0.5 mu g/mL, using 50 mu L serum. The recovery rate exceeded 80.2% for these analytes, except for doripenem (49.1%) and meropenem (62.3%). The present method is applicable to routine therapeutic monitoring of beta-lactam antibiotics in clinical practice. (C) 2011 Elsevier B.V. All rights reserved.