Quantifying the impact of wastewater micronutrient composition on in situ growth activity of Acinetobacter spp.
Quantifying the impact of wastewater micronutrient composition on in situ growth activity of Acinetobacter spp.
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DOI：
10.2166/wst.2002.0514
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发表时间：
2002-01-01
影响因子：
2.7
通讯作者：
Raskin, L
中科院分区：
文献类型：
--
作者：
Oerther, DB;van Loosdrecht, MCM;Raskin, L
采用不同培养基配方对约氏不动杆菌（Acinetobacter johnsonii，T）和约氏不动杆菌210菌株的纯培养物进行分批生长研究，以考察微量营养素组成对废水处理系统中微生物种群生长速率的影响。在营养丰富的Luria-Bertani培养基上，两株A.在以乙酸盐为唯一碳源的基本培养基上，A. johnsonii的倍增时间约为30 min。210 a的倍增时间为2.6h。以人工合成废水为培养基，模式菌株的倍增时间为56 h，菌株210 a的倍增时间为9.62 h。铁的浓度和氧化还原状态似乎是主要的生长限制因素，与二价铁相比，在含有三价铁的介质中发生更高的倍增时间。此外，用靶向不动杆菌属的成熟16 S核糖体RNA（rRNA）和前体16 S rRNA的寡核苷酸杂交探针分析了来自分批生长实验的抓取样品。结果表明，前体16 S rRNA水平的反应更迅速，在更大程度上比总16 S rRNA水平的微量营养素组成的生长介质的变化。前体16 S rRNA水平增加，在这两个菌株过夜培养物用新鲜培养基稀释时，微量营养素补充剂添加到生长的文化。我们的研究结果表明，进水废水的微量营养成分可以有显着的影响，在污水处理系统中的微生物群落结构。
Batch growth studies with pure cultures of Acinetobacter johnsonii(T) and Acinetobacter johnsonii strain 210 on various media formulations were used to examine the effects of micronutrient composition on the growth rate of microbial populations in wastewater treatment systems. On nutrient rich Luria-Bertani medium, both strains of A. johnsonii grew with a doubling time of approximately 30 min. On a defined, minimal medium with acetate as the sole carbon source, the doubling time of A. johnsonii(T) was 9.62 h and the doubling time of strain 210a was 2.6 h. Using a synthetic wastewater as a growth medium, the type strain had a doubling time of 56 h and strain 210a had a doubling time of 9.62 h. The concentration and redox state of iron appeared to be the principle growth limiting factors with higher doubling times occurring in media containing ferric iron as compared to ferrous iron. Additionally, grab samples from batch growth experiments were analyzed with oligonucleotide hybridization probes targeting the mature 16S ribosomal RNA (rRNA) and precursor 16S rRNA of Acinetobacter spp. Results showed that the precursor 16S rRNA levels responded more rapidly and to a greater extent than total 16S rRNA levels to changes in the micronutrient composition of the growth media. Precursor 16S rRNA levels increased in both strains when overnight cultures were diluted with fresh media and when micronutrient supplements were added to growing cultures. Our results show that the micronutrient composition of the influent wastewater can have a significant impact on the microbial community structure in wastewater treatment systems.