Polymerase chain reaction-based differential diagnosis of Ancylostoma duodenale and Necator americanus infections in humans in northern Ghana
Polymerase chain reaction-based differential diagnosis of Ancylostoma duodenale and Necator americanus infections in humans in northern Ghana
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DOI：
10.1111/j.1365-3156.2005.01440.x
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发表时间：
2005-06-01
影响因子：
3.3
通讯作者：
Polderman, AM
中科院分区：
文献类型：
--
作者：
de Gruijter, JM;van Lieshout, L;Polderman, AM
我们评估了一个两步半巢式聚合酶链反应（PCR）为基础的方法，具体检测人类粪便中的钩虫DNA。该试验用于确定在加纳北方的Bolgatanga和Garu地区存在这种钩虫的程度。最初，使用一系列明确定义的对照样品测试PCR的灵敏度和特异性。随后，对来自Bolgatanga和Garu的总共378份人类粪便DNA样本进行了PCR。结果进行了比较，使用以前建立的PCR特异性检测人类粪便中的美洲钩虫DNA。感染A. 74个（19.6%）样品中记录到了微囊藻，N. 278例（73.5%）为美洲种属，其中64例（16.9%）为两种种属的混合感染。而A.在Bolgatanga的样本中主要检测到N.p.，在Garu的感染几乎完全与N.美国人结果表明，该PCR方法是一种有价值的辅助诊断工具。加纳人的疟疾感染，对流行病学研究和监测非洲各区域控制方案的成功具有影响。
We evaluated a two-step semi-nested polymerase chain reaction (PCR)-based approach for the specific detection of Ancylostoma duodenale DNA in human faeces. The test was used to determine to what extent this species of hookworm is present in the regions of Bolgatanga and Garu of northern Ghana. Initially, the sensitivity and specificity of the PCR were tested using a range of well-defined control samples. Subsequently, a total of 378 human faecal DNA samples from Bolgatanga and Garu were subjected to the PCR. The results were compared with those obtained using a previously established PCR for the specific detection of Necator americanus DNA in human faeces. Infection with A. duodenale was recorded in 74 (19.6%) samples and N. americanus in 278 (73.5%), of which 64 (16.9%), represented co-infections with both species. While A. duodenale was predominantly detected in the samples from Bolgatanga, infections in Garu related almost exclusively to N. americanus. The results showed that the present PCR approach is a valuable complementary tool for the diagnosis of A. duodenale infection in humans in Ghana, having implications for epidemiological studies and for the monitoring of the success of control programmes in regions in Africa.