Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
复制标题
DOI：
10.3390/molecules24020322
复制
发表时间：
2019-01-02
期刊：
影响因子：
4.6
通讯作者：
Li, Pengcheng
中科院分区：
文献类型：
--
作者：
Chen, Xiaolin;Song, Lin;Li, Pengcheng
最近，Tribonema sp.（一种丝状微藻）因其积累的三酰基甘油而被研究用于生物燃料生产。然而，Tribonema sp. 的多糖。其生物活性却鲜有报道。在本文中，我们从 Tribonema sp. 中提取了硫酸化多糖。 (TSP)，表征了它们的化学成分和结构，并确定了它们对 RAW264.7 巨噬细胞和 HepG2 细胞的免疫刺激和抗癌活性。结果表明，TSP是一种硫酸化多糖，分子量为197 kDa。 TSP是一种主要由半乳糖组成的杂多糖。它通过刺激巨噬细胞显示出显着的免疫调节活性，例如上调白细胞介素 6 (IL-6)、白细胞介素 10 (IL-10) 和肿瘤坏死因子 (TNF-)。此外，通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)测定，TSP对HepG2细胞也表现出显着的剂量依赖性抗癌活性（250 μg/mL时抑制率高达66.8%）。循环分析表明，TSP的抗癌活性主要是诱导细胞凋亡的结果，而不是影响HepG2细胞的细胞周期和有丝分裂。这些发现表明，TSP 可能具有作为抗癌资源的潜力，但还需要进一步的研究，特别是体内实验，以探索 TSP 的抗癌机制。
Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols. However, the polysaccharides of Tribonema sp. and their biological activities have rarely been reported. In this paper, we extracted sulfated polysaccharides from Tribonema sp. (TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells. The results showed that TSP is a sulfated polysaccharide with a M-w of 197 kDa. TSP is a heteropolysaccharide that is composed mainly of galactose. It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor (TNF-). In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 mu g/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells. These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.