FUNCTIONALLY DISTINCT SUBSETS OF HUMAN GAMMA-DELTA-T-CELLS
FUNCTIONALLY DISTINCT SUBSETS OF HUMAN GAMMA-DELTA-T-CELLS
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DOI：
10.1002/eji.1830211215
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发表时间：
1991-12-01
影响因子：
5.4
通讯作者：
BRENNER, MB
中科院分区：
文献类型：
--
作者：
MORITA, CT;VERMA, S;BRENNER, MB
为了确定人γ/δ T细胞中是否存在效应子亚群，我们检查了具有不同辅助分子表型、V-δ和V-γ基因表达以及J-γ重排的γ/δ T细胞克隆的细胞因子产生和细胞毒活性。带有 γ/δ T 细胞受体的 T 细胞克隆会产生一系列细胞因子，如 α/β T 细胞克隆。单个 γ/δ T 细胞克隆产生一系列特征性细胞因子，与 V-delta 或 V-gamma 基因表达无关。然而，当考虑表型子集时，与 CD4-CD8- 和 CD8+ γ/δ 克隆相比，CD4+ γ/δ 克隆产生显着更高水平的白细胞介素 2 和粒细胞-单核细胞集落刺激因子。同样，当评估细胞毒性潜力时，与 CD4-CD8- 和 CD8+ 成人外周血 γ/δ 克隆相比，CD4+ γ/δ 克隆表现出最小的活性。我们得出结论，存在功能不同的 γ/δ T 细胞亚群，并表明这些亚群可能与 CD4 辅助分子的表达相关。
To determine if effector subsets exist among human gamma/delta T cells, we examined the cytokine production and cytotoxic activity of gamma/delta T cells clones with different accessory molecule phenotypes, V-delta and V-gamma gene expression, and J-gamma rearrangements. T cell clones bearing gamma/delta T cell receptor produce an array of cytokines like alpha/beta T cell clones. Individual gamma/delta T cells clones produced a characteristic array of cytokines without correlation with V-delta or V-gamma gene expression. However, when phenotypic subsets were considered, CD4+ gamma/delta clones produced significantly higher levels of interleukin 2 and granulocyte-monocyte colony-stimulating factor compared with CD4-CD8- and CD8+ gamma/delta clones. Similarly, when cytotoxic potential was assessed, CD4+ gamma/delta clones exhibited minimal activity when compared with CD4-CD8- and CD8+ adult peripheral blood gamma/delta clones. We conclude that functionally distinct gamma/delta T cell subsets exist and suggest that these subsets may correlate with expression of the CD4 accessory molecule.