INFECTION OF SPODOPTERA-FRUGIPERDA CELLS WITH AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS .1. SYNTHESIS OF INTRACELLULAR PROTEINS AFTER VIRUS-INFECTION
INFECTION OF SPODOPTERA-FRUGIPERDA CELLS WITH AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS .1. SYNTHESIS OF INTRACELLULAR PROTEINS AFTER VIRUS-INFECTION
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DOI：
10.1016/0042-6822(79)90017-5
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发表时间：
1979-01-01
期刊：
影响因子：
3.7
通讯作者：
DOERFLER, W
中科院分区：
文献类型：
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作者：
CARSTENS, EB;TJIA, ST;DOERFLER, W
用不同的方法研究了加州核型多角体病毒（AcNPV）在培养的草地贪夜蛾细胞中的复制。感染细胞中的第一个病毒诱导的多肽（分子量为46K[千道尔顿]、30K、29K）在感染后3小时[π]出现。病毒DNA合成在感染后约5小时开始。通过 EM，在感染后 10 小时检测到核内核衣壳，大约在同一时间，细胞内感染性颗粒的滴度开始上升。病毒蛋白质合成的模式相当复杂。在最初的 24 小时内，30-35 个不同的多肽似乎是病毒体的结构蛋白。 28K多角体多肽最初是作为前体合成的，并在转录后进行修饰。多角体蛋白一直合成到感染后期。 AcNPV 复制存在两个不同的阶段：AcNPV 特异性核酸和蛋白质的快速合成以及核衣壳的组装，其中一些通过出芽到细胞外病毒而发育；以及核内膜合成、多面体形成和核内包膜病毒粒子的闭塞。
The replication of A. californica nuclear polyhedrosis virus (AcNPV) in S. frugiperda cells in culture was studied with different methods. The 1st virus-induced polypeptides (with MW of 46K [kilodaltons], 30K, 29K) in infected cells appeared at 3 h postinfection [pi]. Viral DNA synthesis started at about 5 h pi. By EM, intranuclear nucleocapsids were detected at 10 h pi and at about the same time the titer of intracellular infectious particles began to rise. The pattern of viral protein synthesis was rather complex; within the first 24 h 30-35 different polypeptides seemed to be structural proteins of the virion. The 28K polyhedrin polypeptide was synthesized originally as a precursor and was modified posttranscriptionally. Polyhedrin was synthesized until late in infection. Two distinct stages exist in AcNPV replication: the rapid synthesis of AcNPV-specific nucleic acids and proteins and the assembly of nucleocapsids, some of which develop by budding to extracellular virus; and intranuclear membrane synthesis, polyhedra formation and occlusion of intranuclear, enveloped virions.