Novel Streptavidin-Functionalized Silicon Nanowire Arrays for CD4+ T Lymphocyte Separation
Novel Streptavidin-Functionalized Silicon Nanowire Arrays for CD4+ T Lymphocyte Separation
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DOI：
10.1021/nl100942p
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发表时间：
2010-08-01
期刊：
影响因子：
10.8
通讯作者：
Lee, Sang-Kwon
中科院分区：
文献类型：
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作者：
Kim, Sung Tae;Kim, Dong-Joo;Lee, Sang-Kwon
硅纳米线是一种很有前途的无机纳米结构材料。在这里，我们报道了一种新的SiNW阵列的发展，旨在从异质混合细胞群中分离原发CD4(+) T淋巴细胞。我们的系统利用链亲和素（STR）功能化SiNW与生物素标记的CD4(+) T淋巴细胞的特异性高亲和力结合特性。制备的SiNW阵列可以很容易地从小鼠整个脾细胞中分离出CD4(+) T淋巴细胞，其纯度接近88%，扫描电镜显示其与CD4(+) T淋巴细胞紧密附着。因此，我们的STR-SiNW阵列为特定细胞分离提供了一种潜在的工具，并进一步提供了应用于其他生物医学应用领域的可能性。
Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4(+) T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalizeci SiNW with biotin-labeled CD4(+) T lymphocytes. Fabricated SiNW arrays easily separated the CD4(+) T lymphocytes from the mouse whole splenocytes with over similar to 88% purity and demonstrated tight attachment to CD4(+) T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.