Herbicide-resistant sugarcane (Saccharum officinarum L.) plants by Agrobacterium-mediated transformation
Herbicide-resistant sugarcane (Saccharum officinarum L.) plants by Agrobacterium-mediated transformation
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DOI：
10.1007/s004250050369
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发表时间：
1998-09-01
期刊：
影响因子：
4.3
通讯作者：
Selman-Housein, G
中科院分区：
文献类型：
--
作者：
Enríquez-Obregón, GA;Vázquez-Padrón, RI;Selman-Housein, G
甘蔗 (Saccharum officinarum L.) 种植园中不良植物的存在会降低作物产量。使用基因工程作为传统育种方法的补充，可以将抗除草剂性状引入甘蔗种质。对商业除草剂 BASTA 的活性化合物草胺膦 (PPT) 具有抗性的转基因甘蔗植物是通过根癌农杆菌介导的转化产生的。甘蔗的分生组织切片用抗坏死化合物进行处理，以尽量减少氧化爆发，并用作外植体。评估了四种转化方案，转化频率达到10-35%。再生率很高并且似乎不受转化过程的影响。对几种转化植物的Southern印迹分析表明，每个基因组中整合有一个或两个完整拷贝的bar基因，该基因编码PPT乙酰转移酶并赋予对BASTA的抗性。 BASTA 抗性水平在温室和小块土地条件下进行了评估。
The presence of undesirable plants in sugarcane (Saccharum officinarum L.) plantations reduces crop yields. Using genetic engineering as a complement for traditional breeding methods it is possible to introduce herbicide-resistant traits into Saccharum germplasm. Transgenic sugarcane plants resistant to phosphinothricine (PPT), the active compound of the commercial herbicide BASTA were generated by Agrobacterium tumefaciens-mediated transformation. Meristematic sections of sugarcane were treated with anti-necrotic compounds to minimize oxidative bursts and used as explants. Four transformation protocols were assessed and the transformation frequencies reached 10-35%. The regeneration rate was high and did not appear to be affected by the transformation procedure. Southern blot analysis of several transformed plants indicated the integration per genome of one or two intact copies of the bar gene which encodes PPT acetyltransferase and confers resistance to BASTA. The levels of BASTA resistance were evaluated under greenhouse and small-plot conditions.