In vivo white light and contrast-enhanced vital-dye fluorescence imaging of Barrett's-related neoplasia in a single-endoscopic insertion.
In vivo white light and contrast-enhanced vital-dye fluorescence imaging of Barrett's-related neoplasia in a single-endoscopic insertion.
复制标题
单次内窥镜插入中巴雷特相关肿瘤的体内白光和对比增强活体染料荧光成像。
DOI：
10.1117/1.jbo.21.8.086004
复制
发表时间：
2016
影响因子：
3.5
通讯作者：
Richards-Kortum,RebeccaR
中科院分区：
文献类型：
--
作者：
Tang,Yubo;Carns,Jennifer;Polydorides,AlexandrosD;Anandasabapathy,Sharmila;Richards-Kortum,RebeccaR
开发了一种模块化视频内窥镜，用于在单次内窥镜插入中实现白色光成像（WLI）和活性染料荧光成像（VFI），用于早期检测Barrett食管（BE）中的癌症。我们证明，VFI可以实现结合白色光内窥镜，其中适当的白色平衡是用来纠正的存在下的发射过滤器。在VFI模式中，对比度增强功能在真实的时间内实现，以进一步突出BE和相关恶性肿瘤中的腺体模式，而不会引入伪影。在一项初步研究中，我们证明了两种宽视野模式下图像的准确相关性，代表性图像显示了BE中与癌症发展相关的腺体结构的破坏和消失。这些改变的VFI图像表现出增强的对比度相比，WLI。结果表明，VFI在检测BE相关肿瘤中的有用性应在未来的体内研究中进一步评估。
A modular video endoscope is developed to enable both white light imaging (WLI) and vital-dye fluorescence imaging (VFI) in a single-endoscopic insertion for the early detection of cancer in Barrett’s esophagus (BE). We demonstrate that VFI can be achieved in conjunction with white light endoscopy, where appropriate white balance is used to correct for the presence of the emission filter. In VFI mode, a contrast enhancement feature is implemented in real time to further highlight glandular patterns in BE and related malignancies without introducing artifacts. In a pilot study, we demonstrate accurate correlation of images in two widefield modalities, with representative images showing the disruption and effacement of glandular architecture associated with cancer development in BE. VFI images of these alterations exhibit enhanced contrast when compared to WLI. Results suggest that the usefulness of VFI in the detection of BE-related neoplasia should be further evaluated in futurein vivostudies.