The Japanese lamprey (Lethenteron camtschaticum) expresses functional lysophosphatidic acid receptors
The Japanese lamprey (Lethenteron camtschaticum) expresses functional lysophosphatidic acid receptors
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日本七鳃鳗 (Lethenteron camtschaticum) 表达功能性溶血磷脂酸受体
DOI：
10.1016/j.bbrc.2021.06.035
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发表时间：
2021
影响因子：
3.1
通讯作者：
Fukushima Nobuyuki
中科院分区：
文献类型：
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作者：
Tamaki Takeru;Kagawa Nao;Fukushima Nobuyuki
溶血磷脂酸（LPA）信号在哺乳动物和鱼类等脊椎动物的发育过程中发挥着重要作用。七鳃鳗是一种保留了脊椎动物祖先特征的鱼类，但有关七鳃鳗LPA受体基因的信息有限。在这里，利用七鳃鳗基因组数据库中的信息，我们克隆了两个LPA受体基因，Lpar 1和Lpar 5，从日本七鳃鳗（Lethenteron camtschaticum）。七鳃鳗Lpar 1具有较高的氨基酸序列的一致性，小鼠和青鳉鱼Lpar 1，而Lpar 5氨基酸序列的物种之间的差异更大。我们使用异源表达系统的功能分析表明，Lpar 1和Lpar 5响应LPA治疗与G12/13相关的细胞反应，这是细胞骨架作用的指示。日本七鳃鳗中存在功能性LPA受体，表明LPA受体依赖性信号有助于七鳃鳗的生长和发育。
Lysophosphatidic acid (LPA) signaling plays diverse roles in the development of various vertebrates such as mammals and fish. The lamprey is a fish that retains ancestral features of vertebrates, but information regarding lamprey LPA receptor genes is limited. Here, using information from the lamprey genome database, we cloned two LPA receptor genes,Lpar1andLpar5, from the Japanese lamprey (Lethenteron camtschaticum). Lamprey Lpar1 had a high amino acid identity to mouse and medaka fish Lpar1, whereas Lpar5 amino acid sequences were more diverse between species. Our functional analyses using a heterologous expression system demonstrated that Lpar1 and Lpar5 responded to LPA treatment with G12/13-associated cellular responses, which are indicative of cytoskeletal actions. The existence of functional LPA receptors in the Japanese lamprey suggests that LPA receptor-dependent signals contribute to lamprey growth and development.