Strategies to isolate transporters that facilitate organic anion efflux from plant roots
Strategies to isolate transporters that facilitate organic anion efflux from plant roots
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分离促进有机阴离子从植物根部流出的转运蛋白的策略
DOI：
10.1023/a:1022314010798
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发表时间：
2004
期刊：
影响因子：
4.9
通讯作者：
E. Delhaize
中科院分区：
文献类型：
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作者：
P. Ryan;B. Dong;M. Watt;T. Kataoka;E. Delhaize
根系有机阴离子的外排在植物营养中起着重要作用。柠檬酸盐、苹果酸盐和草酸盐等简单羧酸阴离子的释放与铝耐受性和土壤磷获取的改善机制有关。这些代谢物可能作为多价阴离子穿过细胞膜，最近的证据表明，阴离子可渗透通道分别促进了小麦和玉米中苹果酸盐和柠檬酸盐的铝依赖流出。然而，编码这些阴离子通道的基因，或任何其他促进柠檬酸盐、苹果酸盐或草酸盐释放的蛋白质还没有被分离出来。这是生物技术应用于植物抗铝毒性和提高磷获取效率的一个障碍。我们讨论了几种旨在分离促进植物根部有机阴离子释放的基因的策略。
The efflux of organic anions from roots plays an important role in plant nutrition. The release of simple carboxylic anions such as citrate, malate and oxalate have been implicated in mechanisms of aluminium (Al) tolerance and improved acquisition of soil phosphorus. These metabolites are likely to cross cell membranes as multivalent anions and recent evidence indicates that anion-permeable channels facilitate this flow in the Al-dependent efflux of malate and citrate from wheat and maize, respectively. However, the genes encoding these anion channels, or any other protein that facilitates the release of citrate, malate or oxalate have not been isolated. This is an obstacle for the application of biotechnology to combat Al toxicity and to improve P-acquisition efficiency in plants. We discuss several strategies aimed at isolating genes that facilitate organic anion release from plant roots.
Minamikawa, T.：“α-淀粉酶在菜豆和绿豆植物中的表达”Plant Cell Physiol.33 (1992)。
DOI：
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发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
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影响因子：
6.4
作者：
Ezaki, B;Tsugita, S;Matsumoto, H
通讯作者：
Matsumoto, H