Increased urinary folate excretion and decreased plasma folate levels in the rat after acute ethanol treatment.
Increased urinary folate excretion and decreased plasma folate levels in the rat after acute ethanol treatment.
复制标题
急性乙醇治疗后，大鼠尿叶酸排泄增加，血浆叶酸水平降低。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
K. McMartin
中科院分区：
文献类型：
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作者：
K. McMartin
慢性酒精滥用的一个常见并发症是叶酸缺乏症，这可能是由于饮食摄入量不足和乙醇对叶酸代谢的直接影响，如血清叶酸水平的急性下降。向雄性Sprague-Dawley大鼠经口给予乙醇（4 g/kg体重），以研究大鼠作为乙醇急性效应的合适动物模型。通过干酪乳杆菌测定法测定的尿中叶酸的浓度以及尿叶酸排泄量在乙醇给药后4小时显著增加。14小时后，总血浆叶酸水平显著降低至对照水平的50%。在肝脏和红细胞中的总叶酸水平以及通过高压液相色谱法测定的单个肝脏叶酸单谷氨酸衍生物水平中未观察到显著变化。这些结果表明，急性乙醇给药大鼠产生了显着增加尿中的叶酸化合物的排泄，这导致血浆叶酸水平的下降。乙醇处理的大鼠血浆叶酸水平的急性降低与在人类中观察到的相似，表明大鼠是研究乙醇对叶酸代谢的急性影响的合适模型。
A common complication of chronic alcohol abuse is folic acid deficiency, which can result from poor dietary intake and from a direct effect of ethanol on folate metabolism, such as the acute decrease in serum folate levels. Ethanol (4 g/kg body weight) was administered orally to male Sprague-Dawley rats to study the rat as a suitable animal model for the acute effects of ethanol. The concentration of folate in the urine, determined by Lactobacillus casei assay, as well as the amount of urinary folate excretion were markedly increased 4 hr after ethanol administration. After 14 hr, the total plasma folate level was significantly depressed to 50% of control levels. No significant changes were observed in the total folate levels in the liver and red cell nor in the levels of individual hepatic folate monoglutamate derivatives determined by high pressure liquid chromatography assay. These results indicate that acute ethanol administration to rats produces a marked increase in the urinary excretion of folate compounds, which leads to a decrease in plasma folate levels. The acute decrease in plasma folate levels in ethanol-treated rats is similar to that seen in humans and suggests that the rat is an appropriate model for the study of the acute effects of ethanol on folate metabolism.