Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium
Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium
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DOI：
10.1039/b508097e
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发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Thorsen, T
中科院分区：
文献类型：
--
作者：
Vollmer, AP;Probstein, RF;Thorsen, T
本文描述了一种平台，用于实时传感溶解氧在流动的微流体环境中使用的氧敏感的发光染料（铂八乙基卟啉酮）集成到一个微氧合器设备。使用基于相位的检测方法，染料的发光衰减寿命与使用气态和水性样品的线性Stern-Volmer关系一致。最大传感器分辨率在120 - 780 ppb之间变化，在0 - 42.5 ppm的溶解氧（DO）浓度范围内。该传感器随后被用来确定的对流传质特性的多层聚二甲基硅氧烷（PDMS）的微流体氧合器。基于膜的氧合器与分析对流模型显示出极好的一致性，并且集成氧传感器在广泛的测试流速范围内（0.05 - 5 mL min（-1））都是准确的。该设备是独特的，其易于制造和高度灵活的配置，以及新颖的氧气输送和检测在一个单一的微型设备。潜在的应用包括组织工程、细胞培养和小型化生物测定，其需要在微流体构造内递送和/或检测精确量的氧气。
This paper describes a platform for real-time sensing of dissolved oxygen in a flowing microfluidic environment using an oxygen-sensitive luminescent dye ( platinum octaethylporphyrin ketone) integrated into a micro-oxygenator device. Using a phase-based detection method, the luminescent decay lifetime of the dye was consistent with the linear Stern - Volmer relationship using both gaseous and aqueous samples. Maximum sensor resolution varied between 120 780 ppb across a range of dissolved oxygen (DO) concentrations ranging from 0 - 42.5 ppm. The sensor was subsequently used to determine the convective mass-transfer characteristics of a multilayer polydimethylsiloxane (PDMS) microfluidic oxygenator. The membrane-based oxygenator showed excellent agreement with an analytical convection model, and the integrated oxygen sensor was accurate across a wide range of tested flow rates ( 0.05 - 5 mL min(-1)). The device is unique for its ease of fabrication and highly flexible configuration, as well as the novel incorporation of oxygen delivery and detection in a single micro-device. Potential applications include tissue engineering, cell culturing, and miniaturized bio-assays that require the delivery and/or detection of precise quantities of oxygen within a microfluidic construct.