Cell cycle-specific growth inhibitory effect on human gingival fibroblasts of a toxin isolated from the culture medium of Actinobacillus actinomycetemcomitans.
Cell cycle-specific growth inhibitory effect on human gingival fibroblasts of a toxin isolated from the culture medium of Actinobacillus actinomycetemcomitans.
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从伴放线放线杆菌培养基中分离的毒素对人牙龈成纤维细胞的细胞周期特异性生长抑制作用。
DOI：
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发表时间：
1993
影响因子：
3.5
通讯作者：
Øivind Nordby
中科院分区：
文献类型：
--
作者：
Kristen Helgeland;Øivind Nordby
用硫酸铵沉淀法从伴生放线杆菌的生长培养液中分离出一种毒素，该毒素对人牙龈成纤维细胞的增殖有不可逆转的抑制作用。流式细胞仪DNA直方图显示，细胞优先聚集在细胞周期的G2期。这些细胞表现出片状突起，在低浓度毒素处理的细胞中，微核的频率明显增加。经毒素处理的细胞存活数周，台盼蓝和二醋酸荧光素染色显示，通过耗氧量测量的一般细胞代谢没有受到损害。
A toxin isolated from the growth medium of Actinobacillus actinomycetemcomitans by ammonium sulfate precipitation was shown to inhibit irreversibly the multiplication of human gingival fibroblasts. DNA histograms from flow cytometric measurements showed that the cells accumulated preferentially in the G2 phase of the cell cycle. Such cells exhibited sheetlike protrusions, and an increased frequency of micronuclei was evident in cells treated with low concentrations of the toxin. Toxin-treated cells were viable for several weeks, as shown by staining with trypan blue and fluorescein diacetate, and the general cell metabolism as measured by oxygen consumption was unimpaired.
哺乳动物 DNA 拓扑异构酶 II 介导的抗肿瘤吖啶对 DNA 的损伤。
影响因子：
11.2
作者：
Rowe,TC;Chen,GL;Hsiang,YH;Liu,LF
通讯作者：
Liu,LF