Halovulum marinum sp. nov., isolated from deep-sea water of the Indian Ocean, and emended description of the genus Halovulum
Halovulum marinum sp. nov., isolated from deep-sea water of the Indian Ocean, and emended description of the genus Halovulum
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Halovulum marinum sp.
DOI：
10.1099/ijsem.0.004307
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Dai, Xin
中科院分区：
文献类型：
--
作者：
Ruan, Chu-Jin;Wang, Jian;Dai, Xin
从西北印度洋深海水样中分离到一株革兰氏染色阴性、需氧、由周毛鞭毛运动、椭圆形至杆状的细菌，命名为2CG 4（T）。16 S rRNA基因和RpoC蛋白序列的系统发育分析结果表明，该菌株隶属于α-变形菌纲杜鹃花科Amaricoccus Glade的Halovulum属，与Halovulum dunhuangense YYQ-30（T）的模式菌株在16 S rRNA基因水平上的相似性为95.3%。2CG 4（T）的主要脂肪酸（>10%）是特征8（C-18：1 ω 7 c和/或C-18：1 ω 6c;61.1%）和环-C-19：0 ω 8 c（15.6%）的总和。2CG_4（T）的极性脂质为磷脂酰乙醇胺、磷脂酰甲基乙醇胺、磷脂酰甘油、二磷脂酰甘油、磷脂酰胆碱和磺基甘油二酯。2CG 4（T）的唯一类异戊二烯醌是泛醌-10。2CG 4（T）的DNA G+C含量为69.4%。没有检测到光合反应的中心基因pufLM。在不存在NaCl的情况下，2CG 4（T）不发生生长。根据这些数据，我们得出结论，2CG 4（T）代表一个新的物种，该属Halovulum marinum sp. nov.，建议为该物种命名。模式菌株为2CG 4（T）（=CGMCC 1.16468（T）=JCM 32611（T））。
A novel Gram-stain-negative, aerobic, motile by peritrichous flagella, oval to rod-shaped bacterium, designated strain 2CG4(T), was isolated from a deep-sea water sample collected from the Northwest Indian Ocean. The results of phylogenetic analysis of both 16S rRNA gene and RpoC protein sequences indicated that this strain was affiliated with the genus Halovulum in the Amaricoccus Glade of the family Rhodobacteraceae of the class Alphaproteobacteria, sharing 95.3% similarity at the 16S rRNA gene sequence level with the type strain of Halovulum dunhuangense YYQ-30(T), the only species in the genus Halovulum. The predominant fatty acids (>10%) of 2CG4(T) were summed feature 8 (C-18:1 omega 7c and/ or C-18:1 omega 6c;61.1%) and cyclo-C-19:0 omega 8c (15.6%). The polar lipids of 2CG4(T) were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sulfoquinovosyldiacylglycerol. The only isoprenoid quinone of 2CG4(T )was ubiquinone-10. The DNA G+C content of 2CG4(T) was determined to be 69.4%. The central gene pufLM for the photosynthetic reaction was not detected. No growth occurred for 2CG4(T) in the absence of NaCl. On the basis of these data, it is concluded that the 2CG4(T) represents a novel species of the genus Halovulum, for which the name Halovulum marinum sp. nov. is proposed. The type strain is 2CG4(T) (=CGMCC 1.16468(T) =JCM 32611(T)).