Aquaporins and the control of the water status in coffee plants
Aquaporins and the control of the water status in coffee plants
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DOI：
10.1590/s2197-00252013000200001
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发表时间：
2013-01-01
影响因子：
2.6
通讯作者：
Mazzafera, Paulo
中科院分区：
文献类型：
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作者：
Santos, Adriana Brombini dos;Mazzafera, Paulo
在3种咖啡品种(Coffea arabica、C.canephora和Coffemosa)中鉴定了6个水通道蛋白(AQP)同源物，包括4个质膜内源蛋白(PIP1；1，PIP1；2，PIP2；1和PIP2；2)和2个液泡膜内源蛋白(TIP1；1和TIP1；2)。为了更好地了解这些基因在咖啡中的作用，本研究以阿拉比卡咖啡品种Catua和加拿大咖啡品种C.canephora cv.Mundo Novo为材料。Apoata和Mundo Novo在Apoata上的嫁接进行水分胁迫，分析PIP1；2，PIP2；1，PIP2；2和TIP1；2在根和叶中的表达水平。PIP2；1和PIP2；2在叶片和根中的表达明显上调，这表明这些基因可能参与了植物水分状况的调控和灌溉植物的胁迫后恢复。在根中诱导表达最强的是嫁接和未嫁接的Apoata植株。有趣的是，即使在植物浇水后，根中PIP2；1的转录本水平也继续上升，因此不再受到水分胁迫，这表明该基因可能积极参与调控咖啡树根系的水分吸收，特别是在土壤水分不足的情况下。
Six aquaporin (AQP) homologues were identified in three coffee species (Coffea arabica, C. Canephora, and C. racemosa), including four plasma membrane intrinsic proteins (PIP1;1, PIP1;2, PIP2;1, and PIP2;2) and two tonoplast intrinsic proteins (TIP1;1 and TIP1;2). In order to better understand the role of these genes in coffee, C. arabica cvs Catua and Mundo Novo, C. canephora cv. Apoata, and a graft of Mundo Novo on Apoata were water stressed, and the expression levels of PIP1;2, PIP2;1, PIP2;2, and TIP1;2 were analyzed in the roots and leaves. The expression of PIP2;1 and PIP2;2 was clearly up-regulated in the leaves and roots by water deficit, suggesting the possible involvement of these genes in controlling the water status of plants and in the post-stress recovery of irrigated plants. The most strongly induced expression in roots was found in both grafted and non-grafted Apoata plants. Interestingly, the level of PIP2;1 transcripts in roots continued to rise even after the plants were watered and were, therefore, no longer subjected to the water stress, suggesting that this gene may be actively involved in the regulation of water uptake in coffee tree roots, especially when there is a water deficit in the soil.