C1q as a unique player in angiogenesis with therapeutic implication in wound healing
C1q as a unique player in angiogenesis with therapeutic implication in wound healing
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DOI：
10.1073/pnas.1311968111
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发表时间：
2014-03-18
影响因子：
11.1
通讯作者：
Tedesco, Francesco
中科院分区：
文献类型：
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作者：
Bossi, Fleur;Tripodo, Claudio;Tedesco, Francesco
我们以前已经表明，C1 q表达的新形成的蜕膜组织的内皮细胞（ECs）。在这里，我们表明，C1 q沉积在伤口愈合的皮肤中的C4和C3的情况下，C1 q mRNA的局部表达所揭示的实时PCR和原位杂交。在大鼠主动脉环试验中，发现C1 q诱导EC单层的渗透性，刺激EC增殖和迁移，并促进管形成和新血管发芽。使用小鼠伤口愈合模型，我们观察到C1 qa（-/-）小鼠的血管形成有缺陷，局部应用C1 q后恢复正常。C1 q处理的大鼠伤口愈合组织的平均血管密度和愈合的伤口面积显著增加。基于这些结果，我们认为C1 q可能代表一种有价值的治疗剂，可用于治疗慢性溃疡或血管生成受损的其他病理条件，如心肌缺血。
We have previously shown that C1q is expressed on endothelial cells (ECs) of newly formed decidual tissue. Here we demonstrate that C1q is deposited in wound-healing skin in the absence of C4 and C3 and that C1q mRNA is locally expressed as revealed by real-time PCR and in situ hybridization. C1q was found to induce permeability of the EC monolayer, to stimulate EC proliferation and migration, and to promote tube formation and sprouting of new vessels in a rat aortic ring assay. Using a murine model of wound healing we observed that vessel formation was defective in C1qa(-/-) mice and was restored to normal after local application of C1q. The mean vessel density of wound-healing tissue and the healed wound area were significantly increased in C1q-treated rats. On the basis of these results we suggest that C1q may represent a valuable therapeutic agent that can be used to treat chronic ulcers or other pathological conditions in which angiogenesis is impaired, such as myocardial ischemia.