Mechanical stretch upregulates IGFBP-1 secretion from decidualized endometrial stromal cells
Mechanical stretch upregulates IGFBP-1 secretion from decidualized endometrial stromal cells
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DOI：
10.1152/ajpendo.00334.2005
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发表时间：
2006-02-01
影响因子：
5.1
通讯作者：
Taketani, Y
中科院分区：
文献类型：
--
作者：
Harada, M;Osuga, Y;Taketani, Y
脱个体化是子宫内膜分化对胚胎着床和妊娠维持的重要过程。近年来，人们注意到子宫运动引起的机械应力可能对子宫内膜功能有影响。在这项研究中，我们探讨了机械应力对子宫内膜基质细胞（ESC）脱个体化过程的可能影响。ESC在柔性底培养板上培养。在雌二醇和黄体酮作用12天后，在无血清条件下，用计算机操作的细胞张力系统以2周期/分钟的速度，在有或没有循环拉伸（25%伸长）的情况下继续培养24小时。ELISA检测条件培养基中胰岛素样生长因子结合蛋白1 （IGFBP-1）的浓度，RT-PCR检测ESC中IGFBP-1 mRNA的表达。周期拉伸显著增加了去个体化ESC的IGFBP-1分泌。它还增加了去个体化ESC中IGFBP-1 mRNA的表达。放线菌素D能抑制IGFBP-1分泌的增加，而吲哚美辛、PD-98059和H-89不能抑制IGFBP-1的分泌。循环拉伸培养的脱位ESC的条件培养基增加了固定条件下培养的脱位ESC的IGFBP-1分泌。这些发现表明子宫运动调节子宫内膜的去个体化，并对生殖有调节作用。
Decidualization is an essential process of endometrial differentiation for embryo implantation and maintenance of pregnancy. Recently, uterine movement-induced mechanical stress was noticed to have possible effects on endometrial functions. In this study, we addressed the possible effect of mechanical stress on the process of decidualization of endometrial stromal cells ( ESC). ESC were cultured on flexible-bottomed culture plates. After decidualization was achieved with estradiol and progesterone for 12 days, cultures were continued for 24 h with or without cyclic stretch ( 25% elongation) in serum-free conditions at a rate of 2 cycles/min using a computer-operated cell tension system. Concentrations of insulin-like growth factor-binding protein-1 ( IGFBP-1), a marker of decidualization, in the conditioned medium were measured by specific ELISA, and IGFBP-1 mRNA expression in the ESC was measured by RT-PCR. Cyclic stretch remarkably increased IGFBP-1 secretion from decidualized ESC. It also increased IGFBP-1 mRNA in decidualized ESC. The increase in IGFBP-1 secretion was inhibited by actinomycin D but not by indomethacin, PD-98059, or H-89. Conditioned medium of decidualized ESC cultured with cyclic stretch increased IGFBP-1 secretion from decidualized ESC cultured under stationary conditions. These findings imply that uterine movement modulates decidualization of the endometrium and has a regulatory effect on reproduction.