Morphological changes in neurons by self-organized patterned films
Morphological changes in neurons by self-organized patterned films
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DOI：
10.1380/ejssnt.2005.159
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发表时间：
2005
影响因子：
0.7
通讯作者：
Akinori Tsuruma;Masaru Tanaka;N. Fukushima;M. Shimomura
中科院分区：
文献类型：
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作者：
Akinori Tsuruma;Masaru Tanaka;N. Fukushima;M. Shimomura
在组织工程中，微/纳米加工对于修饰基质表面以调节细胞的附着和生长非常重要。在神经科学中，它对于神经再生具有重要意义。这涉及通过充当细胞外基质的细胞培养支架引导和延伸树突和轴突。在这项研究中，我们通过简单的铸造技术制备了高度规则的多孔蜂窝图案薄膜，并培养神经元以研究它们在图案薄膜上的形态。通过扫描电子显微镜和共焦激光扫描显微镜检查神经元的形态。神经元呈圆形，神经突在平膜上随机延伸。这些模式影响神经元的形态。通过改变蜂窝图案薄膜的孔径来改变神经元的形态。神经突沿着蜂窝状图案的边缘扩散。这些结果表明自组织蜂窝图案薄膜是神经组织工程有用的生物材料。 [DOI：10.1380/ejssnt.2005.159]
In tissue engineering, micro/nanofabrication is important to modify substrate surfaces for regulating the attachment and growth of cells. In neuroscience, it is significant for neural regeneration; this involves guiding and extending dendrites and axons by a cell culture scaffold which acts as an extra cellular matrix. In this study, we prepared highly regular porous honeycomb-patterned films by a simple casting technique and cultured neurons to investigate their morphologies on the patterned films. The morphologies of neurons were examined by a scanning electron microscope and a confocal laser scanning microscope. The neurons were round and the neurites extended randomly on the flat film. The patterns influenced the morphologies of neurons. The morphologies of neurons were changed by varying the pore size of the honeycomb- patterned films. The neurites spread along the rims of the honeycomb pattern. These results suggest that the self-organized honeycomb-patterned films are useful biomaterials for neural tissue engineering. [DOI: 10.1380/ejssnt.2005.159]