High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China
High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China
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中国猪和家禽中多重耐药大肠杆菌分离株的高流行和广泛分布
DOI：
10.1016/j.tvjl.2009.10.017
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发表时间：
2011-01-01
影响因子：
2.2
通讯作者：
Zeng, Zhen-Ling
中科院分区：
文献类型：
--
作者：
Jiang, Hong-Xia;Lue, Dian-Hong;Zeng, Zhen-Ling
大肠埃希菌在耐药菌群中扮演着重要的生态角色，可以作为抗菌素耐药性的生物指示剂。本研究的目的是利用大肠埃希菌的这一特性，调查中国地区猪、禽类对抗生素的耐药性及细菌跨物种传播的程度。从猪和家禽(健康和患病动物)中分离的592株大肠埃希菌对8种抗菌药物的22种抗菌素进行了耐药性测试，其中对氨苄西林(99.5%)、多西环素(95.6%)、四环素(93.4%)、甲氧苄氨嘧啶-磺胺甲恶唑(74.3%)、阿莫西林(65.1%)、链霉素(54.7%)和氯霉素(50.2%)的耐药率较高。对头孢菌素、喹诺酮类和氨基糖苷类药物的耐药性也相当普遍。大多数分离株(81%)表现出多重耐药性，最常见的是对5-6种不同的抗菌素类型。其中一株对所有22种抗菌素都具有抗药性。采用脉冲场凝胶电泳法(PFGE)对22株多重耐药菌进行了分析，以评估其在农场之间的分布。发现了三种不同的PFGE类型，表明多重抗菌素耐药细菌在农场之间传播。这项研究证实了中国地区猪和家禽中存在和传播具有多重抗药性的大肠杆菌菌株，并强调了迫切需要适当的监测方案。(C)2009爱思唯尔有限公司。保留所有权利。
Escherichia coli play an important ecological role within resistant bacteria populations, and can be used as a bio-indicator of antimicrobial resistance. The aim of the present study was to use this feature of E. coli to investigate the prevalence of antimicrobial resistance and the degree of cross-species transmission of bacteria in pigs and poultry in China. A total of 592 E. coli strains, isolated from pigs and poultry (healthy and diseased animals), were tested for resistance to 22 antimicrobials representing eight antimicrobial drug types.E. coli isolates had high rates of resistance to ampicillin (99.5%), doxycycline (95.6%), tetracycline (93.4%), trimethoprim-sulfamethoxazole (74.3%), amoxicillin (65.1%), streptomycin (54.7%), and chloramphenicol (50.2%). Resistance to cephalosporins, quinolones, and aminoglycosides was also quite prevalent. The majority (81%) of isolates demonstrated multi-antimicrobial resistance, most commonly to 5-6 different antimicrobial types. One isolate was resistant to all 22 antimicrobials. Twenty-two cultures exhibiting multi-antimicrobial resistance were analysed by pulsed-field gel electrophoresis (PFGE) to assess their distribution between farms. Three distinct PFGE types were identified, indicating inter-farm transmission of multi-antimicrobial-resistant bacteria. The study confirmed the presence and transmission of multi-antimicrobial resistant E. coli strains amongst pigs and poultry in China and highlights the urgent need for appropriate monitoring programmes. (C) 2009 Elsevier Ltd. All rights reserved.